Journal ArticleDOI
Thermodynamic binding studies of galectin-1, -3 and -7.
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TLDR
The results show important differences in the specificities of these three galectins toward poly-N-acetyllactosamine epitopes found on the surface of cells.Abstract:
The carbohydrate binding specificities of the galectin family of animal lectins has been the source of intense recent investigations. Isothermal titration microcalorimetry (ITC) provides direct determination of the thermodynamics of binding of carbohydrates to lectins, and has provided important insights into the fine carbohydrate binding specificities of a wide number of plant and animal lectins. Recent ITC studies have been performed with galectin-1, galectin-3 and galectin-7 and their interactions with sialylated and non-sialylated carbohydrates. The results show important differences in the specificities of these three galectins toward poly-N-acetyllactosamine epitopes found on the surface of cells. Published in 2004.read more
Citations
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Journal ArticleDOI
Characterization of Galectin Fusion Proteins with Glycoprotein Affinity Columns and Binding Assays
TL;DR: In this paper , the authors constructed further fusion proteins of galectins with fluorescent protein color code, and the fusion proteins were purified by affinity chromatography, which revealed an apparent higher binding efficiency for galectin SNAP-tag fusion proteins compared to His6-tagged Galectins, which are independent of the purification mode.
Book ChapterDOI
Evaluation of Galectin Binding by Surface Plasmon Resonance.
TL;DR: This chapter describes the affinity measurement of Galectins-1, -2 and -3 and their glycoside ligands using this approach to study the binding between macromolecules in real time.
Journal ArticleDOI
Galectin-7 reprograms skin carcinogenesis by fostering innate immune evasive programs
Nicolás A. Pinto,Martín Carlos Abba,Lorena Laporte,Juan M. Pérez Sáez,Ada G. Blidner,Nicolás Torres,Rosa M. Morales,Sabrina G. Gatto,Camila Agustina Bach,Florencia Veigas,Hernán García Rivello,Peng Song,Jane H. Frederiksen,Lene Juel Rasmussen,Françoise Poirier,Diego O. Croci,Victoria Sundblad,Gabriel A. Rabinovich,Juan P. Cerliani +18 more
TL;DR: Galectin-7 (Gal-7) was shown to be a critical mediator of skin carcinogenesis and a potential therapeutic target in human non-melanoma skin cancer as discussed by the authors .
Patent
Hybrid galactoside inhibitor of galectins
TL;DR: In this paper, a compound of the general formula (1) which is suitable for use in a method for treating a disorder relating to the binding of a galectin to a ligand in a mammal, such as a human, is presented.
References
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Journal ArticleDOI
A model for p53-induced apoptosis
Kornelia Polyak,Kornelia Polyak,Yong Xia,Jay L. Zweier,Kenneth W. Kinzler,Bert Vogelstein,Bert Vogelstein +6 more
TL;DR: Examination of transcripts induced by p53 expression before the onset of apoptosis stimulated additional biochemical and pharmacological experiments suggesting that p53 results in apoptosis through a three-step process: the transcriptional induction of redox-related genes; the formation of reactive oxygen species; and the oxidative degradation of mitochondrial components, culminating in cell death.
Journal ArticleDOI
Galectins. Structure and function of a large family of animal lectins.
Journal ArticleDOI
Galectins: A Family of Animal Lectins That Decipher Glycocodes
Ken-ichi Kasai,Jun Hirabayashi +1 more
TL;DR: Crystallographic studies revealed that galectins and legume lectins such as concanavalin A have a common topology in spite of the absence of sequence homology, which suggests a possible relationship between animal and plant lectins, and the existence of a lectin super family.
Journal ArticleDOI
X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.
Jayaraman Seetharaman,Amit Kanigsberg,Rita Slaaby,Hakon Leffler,Samuel H. Barondes,James M. Rini +5 more
TL;DR: The x-ray crystal structure of the human galectin-3 CRD, in complex with lactose and N-acetyllactosamine, at 2.1-A resolution was reported in this paper.
Journal Article
Restricted Receptor Segregation into Membrane Microdomains Occurs on Human T Cells During Apoptosis Induced by Galectin-1
TL;DR: It is determined that galectin-1 binds to a restricted set of T cell surface glycoproteins, and that only CD45, CD43, and CD7 appear to directly participate in galectIn-1-induced apoptosis.