Journal ArticleDOI
Tight junctions and the molecular basis for regulation of paracellular permeability
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TLDR
Understanding and manipulating permeability will require a more detailed molecular characterization of tight junction proteins and in particular a characterization of how cell signaling regulates their attachment to the perijunctional cytoskeleton.Abstract:
Tight junctions create a regulated paracellular barrier to the movement of water, solutes, and immune cells between both epithelial and endothelial cells. Recent progress has been made in identifying the proteins that create this barrier. The transmembrane protein occludin is an excellent candidate for the sealing protein and is bound on the cytoplasmic membrane surface to the proteins ZO-1 and ZO-2. Functions for ZO-1 and ZO-2 are suggested by their invertebrate homologues, one of which is a tumor suppressor and another is required in epidermal growth factor receptor signaling. Multiple cellular signaling pathways affect assembly and sealing of junctions. Dynamic regulation of perijunctional actin has emerged as a unifying hypothesis for controlling paracellular permeability. Understanding and manipulating permeability will require a more detailed molecular characterization of tight junction proteins and in particular a characterization of how cell signaling regulates their attachment to the perijunctional cytoskeleton.read more
Citations
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Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to Occludin
TL;DR: It is indicated that multiple integral membrane proteins with four putative transmembrane domains, occludin and claudins, constitute TJ strands.
Journal ArticleDOI
Size-selective loosening of the blood-brain barrier in claudin-5–deficient mice
Takehiro Nitta,Masaki Hata,Shimpei Gotoh,Yoshiteru Seo,Hiroyuki Sasaki,Nobuo Hashimoto,Mikio Furuse,Shoichiro Tsukita +7 more
TL;DR: In claudin-5–deficient mice, the size-selective loosening of the blood-brain barrier was selectively affected, which provides new insight into the basic molecular physiology of BBB and opens a new way to deliver potential drugs across the BBB into the central nervous system.
Journal ArticleDOI
Junctional Adhesion Molecule, a Novel Member of the Immunoglobulin Superfamily That Distributes at Intercellular Junctions and Modulates Monocyte Transmigration
Ines Martin-Padura,Susan Lostaglio,Markus Schneemann,Lisa Williams,Maria Romano,Paolo Fruscella,Carla Panzeri,Antonella Stoppacciaro,Luigi Ruco,Antonello Villa,David Simmons,Elisabetta Dejana +11 more
TL;DR: JAM is a new component of endothelial and epithelial junctions that play a role in regulating monocyte transmigration and is identified as a novel immunoglobulin gene superfamily member that consists of two V-type Ig domains.
Journal ArticleDOI
Claudin-based tight junctions are crucial for the mammalian epidermal barrier a lesson from claudin-1–deficient mice
Mikio Furuse,Masaki Hata,Kyoko Furuse,Yoko Yoshida,Akinori Haratake,Yoshinobu Sugitani,Tetsuo Noda,Tetsuo Noda,Akiharu Kubo,Shoichiro Tsukita +9 more
TL;DR: Findings provide the first evidence that continuous claudin-based TJs occur in the epidermis and that these TJs are crucial for the barrier function of the mammalian skin.
Journal ArticleDOI
Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers.
TL;DR: It is concluded that butyrate enhances the intestinal barrier by regulating the assembly of tight junctions and this dynamic process is mediated by the activation of AMPK.
References
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Journal ArticleDOI
Occludin: a novel integral membrane protein localizing at tight junctions.
TL;DR: An integral membrane protein localizing at tight junctions is now identified, which is designated as "occludin," which was revealed by a hydrophilicity plot that was very similar to that of connexin, an integral membraneprotein in gap junctions.
Journal ArticleDOI
The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling
E. Lowenstein,Roger J. Daly,A. Batzer,W. Li,B. Margolis,Richard L. Lammers,Axel Ullrich,Edward Y. Skolnik,Dafna Bar-Sagi,Joseph Schlessinger +9 more
TL;DR: Results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling.
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A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor
James A. Trofatter,Mia MacCollin,Joni L. Rutter,Jill R. Murrell,Mabel P. Duyao,Dilys M. Parry,Roswell Eldridge,Nikolai Kley,Anil G. Menon,K Pulaski,Volker H. Haase,Christine Ambrose,David J. Munroe,Catherine Bove,Jonathan L. Haines,Robert L. Martuza,Marcy E. MacDonald,Bernd R. Seizinger,M. Priscilla Short,Alan Buckler,James F. Gusella +20 more
TL;DR: A candidate gene for the NF2 tumor suppressor that has suffered nonoverlapping deletions in DNA from two independent NF2 families and alterations in meningiomas from two unrelated NF2 patients is identified.
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Identification of a ten-amino acid proline-rich SH3 binding site
TL;DR: Identification of the SH3 binding site provides a basis for understanding the interaction between the SH2 and SH3 domains and their targets.
Journal ArticleDOI
The discs-large tumor suppressor gene of Drosophila encodes a guanylate kinase homolog localized at septate junctions
Daniel F. Woods,Peter J. Bryant +1 more
TL;DR: The results suggest that a signal transduction process involving guanine nucleotides occurs at the septate junction and is necessary for cell proliferation control in Drosophila epithelia.