Journal ArticleDOI
Treatment of Candida Rugosa Lipase with Short-Chain Polar Organic Solvents Enhances its Hydrolytic and Synthetic Activities
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TLDR
In this paper, a simple and quick treatment based on dissolving the crude lipase from Candida rugosa in different percentages (v/v) of several polar organic solvents was obtained.Abstract:
Following a simple and quick treatment based on dissolving the crude lipase from Candida rugosa in different percentages (v/v) of several polar organic solvents (methanol, ethanol, 1 and 2-propanol, 1 and 2-butanol and acetone) followed by dialysis, different preparations with enhanced activities were obtained. The opening of the lid covering the active site is proposed as the reason for explaining the activity enhancement, both in aqueous and anhydrous organic media.read more
Citations
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Journal ArticleDOI
Production of Biodiesel Using Immobilized Lipase—A Critical Review
TL;DR: This review attempts to provide an updated compilation of the studies reported on biodiesel production by using lipase immobilized through various techniques and the parameters, which affect their functionality.
Journal ArticleDOI
Organic solvent tolerant lipases and applications.
TL;DR: The main emphasis is to study the nature of organic solvent tolerant lipases, and the potential industrial applications that make lipases the biocatalysts of choice for the present and future have been presented.
Journal ArticleDOI
Understanding Candida rugosa lipases: an overview.
TL;DR: In the present paper the recent developments published in the CRL field are overviewed, focusing on comparison of structural and biochemical data among isoenzymes (Lip1-Lip5), and their influence in the biocatalytical performance.
Journal ArticleDOI
Lipase-Catalyzed Esterification
Neena N. Gandhi,Nitin Sopanrao Patil,Sudhirprakash B. Sawant,Jyeshtharaj B. Joshi,Pramod P. Wangikar,D. Mukesh +5 more
TL;DR: This review deals with fundamental as well as practical aspects of lipase catalysis, and various immobilization techniques have been studied for lipases and some have been shown to enhance the activity and stability of the enzyme.
Journal ArticleDOI
The dual roles of phycoremediation of wet market wastewater for nutrients and heavy metals removal and microalgae biomass production
TL;DR: In this article, the dual roles of phycoremediation for nutrients and heavy metals removal and microalgae biomass production are discussed, as well as factors affecting wastewater treatment.
References
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Journal ArticleDOI
A rule to predict which enantiomer of a secondary alcohol reacts faster in reactions catalyzed by cholesterol esterase, lipase from Pseudomonas cepacia, and lipase from Candida rugosa
Romas J. Kazlauskas,Romas J. Kazlauskas,Alexandra N. E. Weissfloch,Aviva Rappaport,Louis A. Cuccia +4 more
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Octanol‐Water Partition Coefficients of Simple Organic Compounds
TL;DR: In this paper, the Octanol Water Particle Coefficients (LWC) for 611 simple organic compounds representing all principal classes have been retrieved from the literature and available experimental details of measurement are documented from original articles.
Journal ArticleDOI
Insights into interfacial activation from an open structure of Candida rugosa lipase.
Pawel Grochulski,Yunge Li,Joseph D. Schrag,Francois Bouthillier,P. Smith,D. Harrison,B. Rubin,Miroslaw Cygler +7 more
TL;DR: The structure of the Candida rugosa lipase determined at 2.06-A resolution reveals a conformation with a solvent-accessible active site and provides a new image of the substrate binding region and active site access, which is different from that inferred from theructure of the "closed" form of the G. candidum lipase.
Journal ArticleDOI
Two conformational states of Candida rugosa lipase.
TL;DR: Comparison of this structure with the previously determined “open” form of this lipase, in which the active site is accessible to the solvent and presumably the substrate, shows that the transition between these 2 states requires only movement of the flap.
Journal ArticleDOI
The solvent dependence of enzyme specificity
TL;DR: The scope, possible mechanisms, and implications of the influence of the reaction medium on enzymatic specificity, as well as directions of future research in the area are discussed.