Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
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This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
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Proceedings ArticleDOI
Femtosecond single optical fiber tweezers enabled two-photon fluorescence excitation of trapped microscopic objects
TL;DR: In this paper, a single fiber femto second optical tweezers and its use in two-photon fluorescence (TPF) excitation of trapped fluorescent particles was reported.
Proceedings ArticleDOI
Real-time imaging of tissue microstructures using intrinsic optical signatures
Bevin Lin,Chad A. Lieber,Jason T. Fitzgerald,Andromachi P. Michalopoulou,Rajesh N. Raman,Christopher D. Pivetti,Christoph Troppmann,Dennis L Matthews,Dennis L Matthews,Stavros G. Demos,Stavros G. Demos +10 more
TL;DR: In this paper, the authors explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design, which utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope.
Proceedings ArticleDOI
Real-time focal modulation microscopy
TL;DR: In this paper, an acoustic optical modulator (AOM) is used to boost the modulation speed and make real-time imaging possible, which can achieve a large penetration depth with a single photon excited fluorescence.
Luminescence lifetime imaging microscopy by confocal pinhole shifting (LLIM-CPS)
Abstract: Venkat K. Ramshesh: Luminescence Lifetime Imaging Microscopy by Confocal Pinhole Shifting (LLIM-CPS) (Under the direction of Dr. John J. Lemasters) Fluorescence lifetime imaging microscopy is a valuable tool for probing biological phenomena independent of luminescence intensity and fluorophore concentrations. Here, I demonstrate an adaptation of a laser scanning confocal microscope (LSCM) for timeresolved lifetime imaging without any add-on equipment. I have named this technique luminescence lifetime imaging microscopy by confocal pinhole shifting (Acronym: LLIM-CPS). I used LLIM-CPS to image europium (Eu) microspheres, a red emitting long lifetime luminescent probe, simultaneously with short life time green-fluorescing microspheres and/or fluorescein and rhodamine in solution. With a one Airy unit pinhole diameter, short lifetime luminescence disappeared rapidly as the pinhole was repositioned in the lagging direction with complete disappearance at one Airy unit distance displacement, whereas long life time luminescence of Eu was retained. In contrast, repositioning the pinhole in the leading or orthogonal directions to the rasting laser spot caused equal loss of short and long lifetime luminescence. These results show the ability of pinhole in the lagging direction to selectively image long lifetime luminescence. By making measurements at 1, 2 and 3 Airy unit lag pinhole positions, lifetime for Eu was
Dissertation
Single Proteins under the Microscope: Conformations, Dynamics and Medicinal Therapies
TL;DR: Single-molecule fluorescence methods were applied to probe the properties of individual fluorescent probes, and to characterize the proteins of interest to which these probes were attached, to investigate heterogeneous subpopulations of the ensemble.