Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
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This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
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Proceedings ArticleDOI
Coherent control of mutiphoton excitation process for biological fluorescence imaging
Katsumi Midorikawa,Jianfan Chen,Hiroyuki Kawano,Yasuo Nabekawa,Akira Suda,Hideaki Mizuno,Atsushi Miyawaki +6 more
TL;DR: In this paper, a two-photon excitation fluorescence (TPEF) process of an enhanced green fluorescent protein (EGFP) for fluorescence signals was adaptively controlled by the phase-modulation of femtosecond pulses.
Optical coherence tomography for structural neuroimaging and non-contact recording of functionally stimulated neural activity
TL;DR: This study demonstrates pr-OCT's capability to detect and measure rapid transient thickness changes in nerves during activity and demonstrates that these transient changes are present at different depths and this allows representing activity as a map of thickness changes.
Proceedings ArticleDOI
Application of two-photon flash photolysis to measure microscopic diffusion and calcium fluxes
TL;DR: An optical system that creates a stationary parfocal TPE uncaging spot on the stage of a conventional confocal microscope is described and the ability of two dyes to track microscopic calcium changes produced by TPE photolysis of DM-nitrophen is examined.
Proceedings ArticleDOI
Advanced SLMs for microscopy
TL;DR: In this article, the authors summarized new spatial light modulators capabilities and benefits for microscopy, and summarized new SLM capabilities, and benefits of using them for image volumetric imaging and programmable excitation.
Proceedings ArticleDOI
Combined Two Photon Excited Fluorescence and Second Harmonic Generation Imaging Microscopy of Collagen Structures
Sotiris Psilodimitrakopoulos,George Filippidis,Christos Kouloumentas,Eleni Alexandratou,Dido Yova +4 more
TL;DR: The main objectives of this work are to combine TPEF and SHG methodologies, in order to elucidate and quantify cross-linking and to describe a model of fibrils orientation within different samples.