Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
About:
This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
Citations
More filters
Journal ArticleDOI
A 0.4-mm-diameter probe for nonlinear optical imaging
Hongchun Bao,Min Gu +1 more
TL;DR: A miniaturized probe that possesses a diameter of 0.4-mm-diamter probe can provide clear two-photon-excited fluorescence images of 10-microm-diameter fluorescent microspheres, close to the diffraction limit.
Journal ArticleDOI
Transplantation into the anterior chamber of the eye for longitudinal, non-invasive in vivo imaging with single-cell resolution in real-time.
TL;DR: In this article, the authors demonstrate the transplantation procedure in the mouse and provide representative results using a model with clinical relevance, namely pancreatic islet transplantation, and provide a platform to screen drugs by performing long-term follow up and monitoring in target tissues.
Journal ArticleDOI
High-throughput spatial light modulation two-photon microscopy for fast functional imaging
Paolo Pozzi,Daniela Gandolfi,Marialuisa Tognolina,Giuseppe Chirico,Jonathan Mapelli,Egidio D'Angelo +5 more
TL;DR: A simpler and fully digital setup (SLM-2PM) which collects three-dimensional two-photon images by only exploiting the SLM leads to an accurate placement of laser beamlets over small focal volumes, eliminating mechanically moving parts and making the system stable over long acquisition times.
Journal ArticleDOI
Application of intravital microscopy in studies of tumor microcirculation
Sarah Jane Lunt,Colin Gray,Constantino Carlos Reyes-Aldasoro,Stephen J. Matcher,Gillian M. Tozer +4 more
TL;DR: Some of the primary advantages and disadvantages of IVM approaches are discussed and recent technological advances in optical microscopy are described with examples of their application to studies of tumor angiogenesis.
Journal ArticleDOI
Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy
Hiroshi Hashimoto,Keisuke Isobe,Akira Suda,Fumihiko Kannari,Hiroyuki Kawano,Hideaki Mizuno,Atsushi Miyawaki,Katsumi Midorikawa +7 more
TL;DR: Measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy are presented.