Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
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This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
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In-Depth Activation of Channelrhodopsin 2-Sensitized Excitable Cells with High Spatial Resolution Using Two-Photon Excitation with a Near-Infrared Laser Microbeam
Samarendra K. Mohanty,Rainer K. Reinscheid,Xiaobin Liu,Naoe Okamura,Tatiana B. Krasieva,Michael W. Berns +5 more
TL;DR: This all-optical method of identification, activation, and detection of ChR2-induced cellular activation in genetically targeted cells with high spatial and temporal resolution will provide a new method of performing minimally invasive in-depth activation of specific target areas of tissues or organisms that have been rendered photosensitive by genetic targeting of Ch R2 or similar photo-excitable molecules.
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A short history of laser development
TL;DR: Years of development since Theodore Maiman's small ruby rod crossed the threshold of laser emission have vastly improved laser performance, tremendously increased their variety, earning lasers important roles in scientific research, consumer products, telecommunications, engineering, medicine, materials working, and a host of other applications.
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Efficiency of resonance energy transfer in homo-oligomeric complexes of proteins.
TL;DR: A theoretical model is proposed for the apparent efficiency of fluorescence (Förster) resonance energy transfer (FRET) in mixtures of free monomers and homo-oligomeric protein complexes of uniform size that provides new insights into the FRET behavior of oligomers comprising more than two monomers.
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Maximum imaging depth of two-photon autofluorescence microscopy in epithelial tissues.
TL;DR: This work demonstrates that the low staining inhomogeneity and large scattering coefficient associated with conventional 2PAM limit the maximum imaging depth to 3 to 5 mean free scattering lengths deep in epithelial tissue.
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Looking at Photoinduced Charge Transfer Processes in the IR: Answers to Several Long-Standing Questions.
TL;DR: The authors' investigations reveal that moderately to highly exergonic reactions result in the formation of both TIPs and LIPs, and a transition from a purely quadrupolar state to a symmetry-broken state on the timescale of solvent relaxation could be clearly observed in polar solvents, indicating that symmetry breaking occurs primarily via solvent fluctuations.