Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
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This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
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Conjugated-polymer-based red-emitting nanoparticles for two-photon excitation cell imaging with high contrast.
TL;DR: Red-emitting-dye-doped conjugated polymer nanoparticles that display high two-photon excitation brightness are reported that could become a general approach to the preparation of two- phytochemical excitation red-emitter materials for deep-tissue live-cell imaging with high contrast.
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Intravital two-photon microscopy: focus on speed and time resolved imaging modalities.
TL;DR: Current available two‐photon microscopy techniques are described with a focus on novel approaches that allow very high image acquisition rates compared with state‐of‐the‐art systems, and the analysis of endogenous fluorophores such as NAD(P)H as a way to analyze the redox status in cells with subcellular resolution.
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Calibration of a wide‐field frequency‐domain fluorescence lifetime microscopy system using light emitting diodes as light sources
TL;DR: The results show that phase lifetime measurements are robust down to sub ns levels, whereas modulation lifetimes are prone to errors even at large signal‐to‐noise ratios, and strategies for optimizing measurement fidelity are discussed.
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Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells
Clément Ricard,Erica D. Arroyo,Cynthia X. He,Carlos Portera-Cailliau,Gabriel Lepousez,Marco Canepari,Daniel Fiole +6 more
TL;DR: This review compile and homogenize the two-photon spectral properties of 280 fluorescent probes and discusses different approaches, strategies, and prerequisites for two- photon multicolor experiments in the brains of living laboratory animals.
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Video-rate resonant scanning multiphoton microscopy: An emerging technique for intravital imaging of the tumor microenvironment
Nathaniel D. Kirkpatrick,Euiheon Chung,Daniel C. Cook,Xiaoxing Han,Gabriel Gruionu,Shan Liao,Lance L. Munn,Timothy P. Padera,Dai Fukumura,Rakesh K. Jain +9 more
TL;DR: The development and implementation of a video-rate multiphoton microscope (VR-MPLSM) based on resonant galvanometer mirror scanning that is capable of recording at 30 frames per second and acquiring intravital multispectral images is reported, and the design can be readily implemented and is adaptable to various experimental models.