Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
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This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
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Imaging mitochondrial dynamics in human skin reveals depth-dependent hypoxia and malignant potential for diagnosis
Dimitra Pouli,Mihaela Balu,Carlo Amadeo Alonzo,Zhiyi Liu,Kyle P. Quinn,Kyle P. Quinn,Francisca Rius-Diaz,Ronald M. Harris,Kristen M. Kelly,Bruce J. Tromberg,Irene Georgakoudi +10 more
TL;DR: It is reported that mitochondrial dynamics can be monitored in vivo, within intact human skin by relying entirely on endogenous two-photon–excited fluorescence from the reduced metabolic coenzyme nicotinamide adenine dinucleotide (NADH).
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Towards large scale CMOS single-photon detector arrays for lab-on-chip applications
TL;DR: The miniaturization and performance potential of solid-state single-photon detectors are discussed in the context of lab-on-chip applications where high accuracy and/or high levels of parallelism are suited.
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Fluorescence Techniques Using Dehydroergosterol to Study Cholesterol Trafficking
Avery L. McIntosh,Barbara P. Atshaves,Huan Huang,Adalberto M. Gallegos,Ann B. Kier,Friedhelm Schroeder +5 more
TL;DR: The properties and behavior of DHE in protein-binding, lipoproteins, model membranes, biological membranes, lipid rafts/caveolae, and real-time imaging in living cells indicate that this naturally occurring fluorescent sterol is a useful mimic for probing the properties of cholesterol in these systems.
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Breaking the spatial resolution barrier via iterative sound-light interaction in deep tissue microscopy
Ke Si,Reto Fiolka,Meng Cui +2 more
TL;DR: Fluorescence microscopy beyond the ballistic regime of light is demonstrated with a threefold improved resolution and a fivefold increase in contrast and opens up practical high resolution fluorescence imaging in deep tissues.
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Two-photon excited hemoglobin fluorescence
TL;DR: It is discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources, enabling two- photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.