Open AccessJournal Article
[Two-photon laser scanning fluorescence microscopy].
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This article is published in Tanpakushitsu kakusan koso. Protein nucleic acid enzyme.The article was published on 2007-10-01 and is currently open access. It has received 1480 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Microscopy.read more
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Journal ArticleDOI
Local versus global scales of organization in auditory cortex.
TL;DR: Drawing from various cortical areas, cortical layers, recording methodologies, and species, it is described how auditory cortical circuitry can simultaneously support a globally systematic, yet locally heterogeneous representation of this fundamental sound property.
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The Power of Single and Multibeam Two-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging
TL;DR: A multifocal scanhead splitting a laser beam into a line of 64 foci is utilized, allowing sample illumination in real time at full laser power, and it is found that the point-spread function of both devices strongly depends on tissue constitution and penetration depth.
Journal ArticleDOI
Quantitative assessment of collagen fibre orientations from two-dimensional images of soft biological tissues
Andreas Jörg Schriefl,Andreas J. Reinisch,Sethuraman Sankaran,David M. Pierce,Gerhard Holzapfel,Gerhard Holzapfel +5 more
TL;DR: This work outlines an automated method for the extraction and quantification of material parameters characterizing collagen fibre orientations from two-dimensional images that provides an ideal tool for quickly extracting mechanically relevant tissue parameters which have implications for computational modelling and can also be used for the inhomogeneous modelling of tissues.
Book ChapterDOI
Aberrations in confocal and multi-photon fluorescence microscopy induced by refractive index mismatch
Alexander Egner,Stefan W. Hell +1 more
TL;DR: In this paper, the authors assume that the manufacturer does not intend to discourage purchase by emphasizing the pitfalls that unavoidably arise from the physics of imaging, and they assume that typical users may be unaware of the very existence of such limitations either because they may unwittingly work within the limits or because they fail to recognize their effects.
Journal ArticleDOI
Advanced Fiber Soliton Sources for Nonlinear Deep Tissue Imaging in Biophotonics
TL;DR: In this article, the basics of deep tissue multiphoton microscopy and its improvements utilizing soliton self-frequency shift (SSFS) are reviewed, and the application of these solitons to MPM enables noninvasive imaging in biological tissues with unprecedented depth.