Journal ArticleDOI
Typing of Hantaviruses from five continents by polymerase chain reaction
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TLDR
PCR can be used as an adjunct test with serological method(s) or an independent test for diagnosis and for typing of new isolates of Hantaviruses.About:
This article is published in Virus Research.The article was published on 1992-10-01. It has received 41 citations till now. The article focuses on the topics: Typing.read more
Citations
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Journal ArticleDOI
Molecular Typing of Enteroviruses: Current Status and Future Requirements
Peter Muir,Ulrike Kämmerer,Klaus Korn,Mick N. Mulders,Tuija Pöyry,Benedikt Weissbrich,Reinhard Kandolf,Graham M. Cleator,Anton M. van Loon +8 more
TL;DR: The case for developing a molecular typing system is argued, the genetic basis of such a system is discussed, the literature describing attempts to identify or classify enteroviruses by molecular methods is reviewed, and ways in which the goal of molecular typing may be realized are suggested.
Journal ArticleDOI
Genetic and antigenic properties of Dobrava virus: a unique member of the Hantavirus genus, family Bunyaviridae
TL;DR: Phylogenetic comparisons of the M and S segments ofDOB virus to those of other hantaviruses indicated that DOB virus is similar to, but clearly distinct from Hantaan (HTN) and Seoul (SEO) viruses.
Journal ArticleDOI
Isolation, characterization and geographic distribution of Caño Delgadito virus, a newly discovered South American hantavirus (family Bunyaviridae)
Charles F. Fulhorst,Martha C. Monroe,Rosa Alba Salas,Gloria Duno,Antonio Utrera,Thomas G. Ksiazek,Stuart T. Nichol,Nuris de Manzione,Duilia Tovar,Robert B. Tesh +9 more
TL;DR: The results of serological tests and analyses of small and medium RNA segment nucleotide sequence data indicated that VHV-574 represents a novel hantavirus (proposed name 'Caño Delgadito') that is distinct from all previously characterized hantviruses.
Book ChapterDOI
Epidemiology and Pathogenesis of Hemorrhagic Fever with Renal Syndrome
TL;DR: The outbreak of deadly hantavirus pulmonary syndrome in the United States in 1993 changed the concept of hemorrhagic fever with renal syndrome (HFRS) because the clinical manifestations are quite different from the known typical forms of HFRS caused by Hantaan, Seoul, and Puumala viruses.
Journal ArticleDOI
Puumala Hantavirus Viremia Diagnosed by Real-Time Reverse Transcriptase PCR Using Samples from Patients with Hemorrhagic Fever and Renal Syndrome
Magnus Evander,Irene Eriksson,Lisa Pettersson,Per Juto,Clas Ahlm,Gert E. Olsson,Göran Bucht,Annika Allard +7 more
TL;DR: It is demonstrated that real-time RT-PCR is a useful method for diagnosis of PUUV viremia and for detecting PUUV RNA at early time points, before the appearance of IgM antibodies.
References
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Journal ArticleDOI
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
Journal ArticleDOI
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer
TL;DR: The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.
Hemorrhagic fever with renal syndrome.
Ho Wang Lee,G van der Groen +1 more
TL;DR: Based on the observation of 11 patients (10 males and 1 female), the occurrence of hemorrhagic fever with renal syndrome in two new geographic areas of Romania is reported, two patients died within several hours after admission and four patients recovered gradually.
Journal ArticleDOI
Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order
TL;DR: The M genome segment of Hantaan virus was molecularly cloned and the nucleotide sequence of cDNA was determined, revealing a gene order with respect to message sense RNA of 5'-G1-G2-3'.
Journal ArticleDOI
Coding strategy of the S genome segment of Hantaan virus.
TL;DR: The data demonstrate that the coding strategy of the Hantaan virus S RNA is different than those reported for other viruses in this family.