Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae
Wenqing Shui,Yun Xiong,Weidi Xiao,Xianni Qi,Yong Zhang,Yuping Lin,Yufeng Guo,Zhidan Zhang,Qinhong Wang,Yanhe Ma +9 more
TLDR
The authors' proteomic characterization of the industrial strain led to comprehensive understanding of the molecular basis of thermotolerance, which would facilitate future improvement in the industrially important trait of S. cerevisiae by rational engineering.About:
This article is published in Molecular & Cellular Proteomics.The article was published on 2015-07-01 and is currently open access. It has received 55 citations till now. The article focuses on the topics: Heat shock.read more
Citations
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Thermotolerant fermenting yeasts for simultaneous saccharification fermentation of lignocellulosic biomass
TL;DR: This review describes the various approaches to screen and develop thermotolerant yeasts via genetic and metabolic engineering and the advantages and limitations of SSF at high temperatures, and a critical insight into the effect of high temperatures on yeast morphology and physiology is included.
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Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis
TL;DR: It is argued for a new approach that leverages the native stress-tolerant phenotypes of non-conventional microbes that directly address design challenges from the outset to enable more efficient bioprocessing for chemical production.
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Bioprospecting thermotolerant ethanologenic yeasts for simultaneous saccharification and fermentation from diverse environments
TL;DR: S. cerevisiae JRC6, based on its ability to ferment sugars at a higher temperature, can be a promising candidate for production of ethanol from lignocellulosic biomass via SSF process.
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Next-generation proteomics faces new challenges in environmental biotechnology.
TL;DR: Next-generation proteomics faces new, interesting challenges when the organism is distantly related to previously characterized organisms or when mixtures of organisms have to be analyzed.
References
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Universal sample preparation method for proteome analysis
TL;DR: A method is described, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics and allows single-run analyses of organelles and an unprecedented depth of proteome coverage.
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Audrey P. Gasch,Paul T. Spellman,Camilla M. Kao,Orna Carmel-Harel,Michael B. Eisen,Gisela Storz,David Botstein,Patrick O. Brown +7 more
TL;DR: Analysis of genomic expression patterns in the yeast Saccharomyces cerevisiae implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators.
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Molecular Chaperones in the Cytosol: from Nascent Chain to Folded Protein
TL;DR: Understanding how the thousands of different proteins synthesized in a cell use this chaperone machinery has profound implications for biotechnology and medicine.
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Remodeling of Yeast Genome Expression in Response to Environmental Changes
Helen C. Causton,Bing Ren,Sang Seok Koh,Christopher T. Harbison,Elenita I. Kanin,Ezra G. Jennings,Tong Ihn Lee,Heather L. True,Eric S. Lander,Richard A. Young +9 more
TL;DR: The results of expression analysis with MSN2/MSN4 mutants support the model that the Msn2/Msn4 activators induce the common response to environmental change and extend the understanding of the role of activators in effecting this response.