Journal ArticleDOI
Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1-GAL10 genes.
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TLDR
Although sequences protected by histones in the repressed state became more accessible to micrococcal nuclease and (methidiumpropyl-EDTA)iron(II) cleavage following induction of transcription, DNA-protein particles containing these sequences retained the electrophoretic mobility of nucleosomes, indicating that the promoter region can be associated with nucleosome under conditions of transcription activation.Abstract:
Conversion of the positioned nucleosome array characteristic of the repressed GAL1-GAL10 promoter region to the more accessible conformation of the induced state was found to depend on the upstream activation sequence, GAL4 protein, a positive regulator of transcription, and galactose, the inducing agent. The effect of the GAL4 protein-upstream activation sequence complex on the structure of adjacent chromatin required no other promoter sequences. Although sequences protected by histones in the repressed state became more accessible to micrococcal nuclease and (methidiumpropyl-EDTA)iron(II) cleavage following induction of transcription, DNA-protein particles containing these sequences retained the electrophoretic mobility of nucleosomes, indicating that the promoter region can be associated with nucleosomes under conditions of transcription activation.read more
Citations
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Evidence for nucleosome depletion at active regulatory regions genome-wide.
TL;DR: Evidence is presented that the basic repeating units of eukaryotic chromatin, nucleosomes, are depleted from active regulatory elements throughout the Saccharomyces cerevisiae genome in vivo and the level of nucleosome occupancy is inversely proportional to the transcriptional initiation rate at the promoter.
Journal ArticleDOI
Alternative chromatin structure at CpG islands.
Jamal Tazi,Adrian Bird +1 more
TL;DR: CpG island chromatin differs in three respects from bulk chromatin prepared in the same way: histone H1 is present in very low amounts; histones H3 and H4 are highly acetylated; and nucleosome-free regions are present.
Journal ArticleDOI
Variant histone H2A.Z is globally localized to the promoters of inactive yeast genes and regulates nucleosome positioning.
Benoit Guillemette,Alain R. Bataille,Nicolas Gévry,Maryse Adam,Mathieu Blanchette,François Robert,Luc Gaudreau +6 more
TL;DR: The data suggest that the incorporation of H2A.Z into specific promoter-bound nucleosomes configures chromatin structure to poise genes for transcriptional activation, and the relevance of these findings to higher eukaryotes is discussed.
Journal ArticleDOI
Transcriptional regulation in the yeast GAL gene family: a complex genetic network.
D Lohr,P Venkov,J Zlatanova +2 more
TL;DR: The mechanisms which control structural and regulatory gene expression in the GAL family are compared and GAL structural/regulatory gene chromatin structure is discussed.
Journal ArticleDOI
Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II
TL;DR: In vitro transcription analysis of chromatin reconstituted in the absence or presence of histone H1 provides direct biochemical evidence that support and clarify previously proposed models in which there is depletion or reconfiguration of nucleosomal cores and hist one H1 at the promoter regions of active genes.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Transformation of intact yeast cells treated with alkali cations.
TL;DR: The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Journal ArticleDOI
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
Arnold J. Berk,Phillip A. Sharp +1 more
TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
Journal ArticleDOI
Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase
Marian Carlson,David Botstein +1 more
TL;DR: A model is proposed to account for the synthesis and regulation of the two forms of inverts: the larger, regulated mRNA contains the initiation codon for the signal sequence required for synthesis of the secreted, glycosylated form of invertase; the smaller, constitutively transcribed mRNA begins within the coding region of the signal sequences, resulting in synthesis ofThe intracellular enzyme.