Journal ArticleDOI
YidC mediates membrane protein insertion in bacteria.
James C. Samuelson,Minyong Chen,Fenglei Jiang,Ines Möller,Martin Wiedmann,Andreas Kuhn,Gregory J. Phillips,Ross E. Dalbey +7 more
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TLDR
It is shown that membrane insertion of two Sec-independent proteins requires YidC, which is essential for E. coli viability and homologues are present in mitochondria and chloroplasts.Abstract:
The basic machinery for the translocation of proteins into or across membranes is remarkably conserved from Escherichia coli to humans. In eukaryotes, proteins are inserted into the endoplasmic reticulum using the signal recognition particle (SRP) and the SRP receptor, as well as the integral membrane Sec61 trimeric complex (composed of alpha, beta and gamma subunits). In bacteria, most proteins are inserted by a related pathway that includes the SRP homologue Ffh, the SRP receptor FtsY, and the SecYEG trimeric complex, where Y and E are related to the Sec61 alpha and gamma subunits, respectively. Proteins in bacteria that exhibit no dependence on the Sec translocase were previously thought to insert into the membrane directly without the aid of a protein machinery. Here we show that membrane insertion of two Sec-independent proteins requires YidC. YidC is essential for E. coli viability and homologues are present in mitochondria and chloroplasts. Depletion of YidC also interferes with insertion of Sec-dependent membrane proteins, but it has only a minor effect on the export of secretory proteins. These results provide evidence for an additional component of the translocation machinery that is specialized for the integration of membrane proteins.read more
Citations
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Versatility of the mitochondrial protein import machinery
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References
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Book
Cell biology :a laboratory handbook.
TL;DR: This volume discusses cell and Tissue Culture: Associated Techniques, which focuses on culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial, and concludes with a discussion of Transgenic, Knockouts and knock-down methods.
Journal ArticleDOI
New method for generating deletions and gene replacements in Escherichia coli.
TL;DR: A method for generating gene replacements and deletions in Escherichia coli using a temperature-sensitive pSC101 replicon to facilitate the gene replacement and can be used to generate deletions of essential genes.
Journal ArticleDOI
Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes
TL;DR: Key components of the translocated apparatus have now been identified and the translocation pathways seem likely to be related to each other but mechanistically distinct.
Journal ArticleDOI
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
Pier A. Scotti,Malene L. Urbanus,Josef Brunner,Jan-Willem de Gier,Gunnar von Heijne,Chris van der Does,Arnold J. M. Driessen,Bauke Oudega,Joen Luirink +8 more
TL;DR: It is proposed that YidC, homologous to Saccharomyces cerevisiae Oxa1p, is involved in the insertion of hydrophobic sequences into the lipid bilayer after initial recognition by the SecAYEG translocase.
Journal ArticleDOI
The E. coli Signal Recognition Particle Is Required for the Insertion of a Subset of Inner Membrane Proteins
TL;DR: Inhibition of the SRP pathway sharply blocked the membrane insertion of several polytopic inner membrane proteins (IMPs) that were predicted to be SRP substrates, but had a smaller effect on the insertion of other IMPs and no significant effect on preprotein translocation.