Journal ArticleDOI
Zinc Inhibits Collagenolysis by Cathepsin K and Matrix Metalloproteinases in Demineralized Dentin Matrix.
TLDR
Cathepsin K-induced dentin collagen degradation can be strongly inhibited by zinc, and zinc levels of ≥5 mM can be considered as a reliable threshold for the stabilization of dentin matrices.Abstract:
The enzymatic degradation of dentin organic matrix occurs via both the action of matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). Zinc can prevent collagen hydrolysis by MMPs. However, its effect on the activity of dentin-bound CCs is not known. The aim of this study was to investigate the effect of zinc on matrix-bound cathepsin K and MMP activity in dentin. Completely demineralized dentin beams were divided into test groups (n = 9) and incubated at 37°C in an incubation media (1 mL) containing ZnCl2 of 0.02 (physiological level, control), 0.2, 0.5, 1, 5, 10, 20, 30, or 40 mM. The dry mass changes of the beams were determined, and incubation media were analyzed for cathepsin K- and MMP-specific collagen degradation end products - CTX (C-terminal cross-linked telopeptide of type I collagen) and ICTP (cross-linked carboxy-terminal telopeptide of type I collagen) - at 1, 3, and 7 days of incubation. The mass loss of the beams decreased when the zinc level in the incubation media was ≥5 mM (p < 0.05). The release of liberated collagen degradation telopeptides decreased in accordance with the decrease in the mass loss rates of the beams. Cathepsin K-induced dentin collagen degradation can be strongly inhibited by zinc. Zinc levels of ≥5 mM can be considered as a reliable threshold for the stabilization of dentin matrices.read more
Citations
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Journal ArticleDOI
Stannous chloride and stannous fluoride are inhibitors of matrix metalloproteinases
Barbara Cvikl,Barbara Cvikl,Adrian Lussi,Thiago Saads Carvalho,Andreas Moritz,Reinhard Gruber +5 more
TL;DR: Inhibition of MMPs using SnCl2 and SnF2 could play an important role in the prevention of dental erosion and caries, and the clinical relevance of these findings needs to be proven.
Journal ArticleDOI
The effects of zinc treatment on matrix metalloproteinases: A systematic review.
TL;DR: The inhibitory or stimulatory effect of Zn on MMPs seems to depend on physiological conditions of the cells or animals used, dose ofZn used, and duration of treatment.
Journal ArticleDOI
Zinc-Calcium-Fluoride Bioglass-Based Innovative Multifunctional Dental Adhesive with Thick Adhesive Resin Film Thickness.
Chenmin Yao,Chenmin Yao,Mohammed H Ahmed,Mohammed H Ahmed,Xin Li,Ivana Nedeljkovic,Ivana Nedeljkovic,Jennifer Vandooren,Ben Mercelis,Fei Zhang,Kirsten Van Landuyt,Cui Huang,Bart Van Meerbeek +12 more
TL;DR: An experimental three/two-step E&R/SE Exp_2UA combines high bonding potential and bond-degradation resistance with long-term ion release rendering the adhesive anti-enzymatic and anti-bacterial potential.
Journal ArticleDOI
Effects of silver diamine fluoride/potassium iodide on artificial root caries lesions with adjunctive application of proanthocyanidin.
TL;DR: In this in vitro study, cross-linking agents were applied with a fluoride regimen, which improved its treatment efficacy of root caries regarding the distribution of ion uptake and recovery of dentine biomechanics.
Journal ArticleDOI
A novel prime-&-rinse mode using MDP and MMPs inhibitors improves the dentin bond durability of self-etch adhesive
Jingqiu Xu,Mingxing Li,Wenting Wang,Zhifang Wu,Chaoyang Wang,Jin Xiaoting,Ling Zhang,Wenxiang Jiang,Baiping Fu +8 more
TL;DR: The novel prime-&-rinse mode using 5%M DP-BAC, 15%MDP and 15% MDP+MMPs inhibitors could significantly increase the short- and long-term dentin MTBS of self-etch adhesive, and might be a supplement to contemporary dentin bonding strategies.
References
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Journal ArticleDOI
Role of nutritional zinc in the prevention of osteoporosis
TL;DR: The oral administration of AHZ or zinc acexamate has the restorative effect on bone loss under various pathophysiologic conditions including aging, skeletal unloading, aluminum bone toxicity, calcium- and vitamin D-deficiency, adjuvant arthritis, estrogen deficiency, diabetes, and fracture healing.
Journal ArticleDOI
Cysteine cathepsins and extracellular matrix degradation
TL;DR: Cysteine cathepsins are among the major proteases involved in ECM remodeling, and their role is not limited to degradation only, and they are now validated targets in a number of ECM-linked diseases.
Journal ArticleDOI
Ecological Hypothesis of Dentin and Root Caries.
Nobuhiro Takahashi,Bente Nyvad +1 more
TL;DR: The detection of proteolytic/amino acid-degrading bacteria and bacterial metabolites in dentin and root caries suggests a bacterial digestion and metabolism of partly degraded matrix, which might induce pulpitis as an inflammatory/immunomodulatory factor.
Journal ArticleDOI
Cysteine Cathepsins in Human Dentin-Pulp Complex
Ivarne L.S. Tersariol,Saulo Geraldeli,Christiane L. Minciotti,Fabio D. Nascimento,Virve Pääkkönen,Marília Trierveiler Martins,Marcela Carrilho,David H. Pashley,Franklin R. Tay,Tuula Salo,Tuula Salo,Leo Tjäderhane,Leo Tjäderhane +12 more
TL;DR: Cathepsin and MMP activities were observed in all dentin samples, with significant negative correlations in their activities with tooth age, and their role, along with MMPs, in dentin modification with aging is suggested.
Journal ArticleDOI
The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices
Arzu Tezvergil-Mutluay,Arzu Tezvergil-Mutluay,Kelli A. Agee,Tomohiro Hoshika,Marcela Carrilho,Lorenzo Breschi,Leo Tjäderhane,Yoshihiro Nishitani,Ricardo M. Carvalho,Stephen W. Looney,Franklin Chi Meng Tay,David H. Pashley +11 more
TL;DR: The storage solutions used to age resin-dentin bonds should be buffered solutions that contain both calcium and zinc and the common use of water as an aging medium may underestimate the hydrolytic activity of endogenous dentin MMPs.