Andrew D. Ellington

TL;DR: In this paper , a combined screening and selection approach was developed to refine the affinities and specificities of generalist transcription factors; using RamR as a starting point, they evolve highly specific (>100-fold preference) and sensitive (half-maximum effective concentration (EC50) < 30 μM) biosensors for the alkaloids tetrahydropapaverine, papaverine and noscapine.

TL;DR: The results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acids circuitry.

TL;DR: Using Rosetta to guide the introduction of an oxidizable crosslinking NCAA, l-3,4-dihydroxyphenylalanine (l-DOPA), into the CDRs of the anti-protective antigen scFv antibody M18, the results suggest that computational analysis can be used in a pipeline for engineering crossl linking antibodies.

TL;DR: An in vitro autogene composed of a self-amplifying T7 RNA polymerase system that amplifies the autogene genetic information through a positive feedback architecture is described.

TL;DR: The single-strand siRNA anions were observed to dissociate via cleavage of the 5′ P—O bonds yielding c- and y-type product ions as well as undergo neutral base loss, similar to the dissociation trends observed for duplex DNA.