A
Andrew D. Ellington
Researcher at University of Texas at Austin
Publications - 599
Citations - 48723
Andrew D. Ellington is an academic researcher from University of Texas at Austin. The author has contributed to research in topics: Aptamer & RNA. The author has an hindex of 96, co-authored 569 publications receiving 43262 citations. Previous affiliations of Andrew D. Ellington include Harvard University & UPRRP College of Natural Sciences.
Papers
More filters
Reference EntryDOI
In vitro selection of RNA aptamers to a small molecule target.
TL;DR: This unit describes the selection of aptamers that bind to a small molecule target from a single‐stranded RNA pool with two modes of selection, one by column filtration and one by batch selection.
Journal ArticleDOI
Directed evolution of the substrate specificity of biotin ligase
TL;DR: A developed selection scheme for directing the evolution of Escherichia coli biotin protein ligase (BPL) via in vitro compartmentalization, and has used this scheme to alter the substrate specificity of the ligase towards the utilization of the biotin analogue desthiobiotin.
Journal ArticleDOI
Chemical functionalization of oligodeoxynucleotides with multiple boronic acids for the polyvalent binding of saccharides.
Amanda E. Hargrove,Andrew D. Ellington,Eric V. Anslyn,Jonathan L. Sessler,Jonathan L. Sessler +4 more
TL;DR: A requirement for multiple recognition sites in these conjugate systems is identified and the results are expected to facilitate future efforts toward applying synthetic recognition systems to the realm of macromolecules.
Book ChapterDOI
Nucleic acid pool preparation and characterization.
TL;DR: The following protocol takes the reader through the steps necessary for the preparation of a pool of known complexity of random sequence nucleic acids.
Journal ArticleDOI
A facile technology for the high-throughput sequencing of the paired VH:VL and TCRβ:TCRα repertoires.
Hidetaka Tanno,Jonathan R. McDaniel,Christopher A. Stevens,William N. Voss,Jie Li,Russell E. Durrett,Jiwon Lee,Jiwon Lee,Jimmy Gollihar,Jimmy Gollihar,Yuri Tanno,George Delidakis,Arti Pothukuchy,Jared W. Ellefson,Jörg J. Goronzy,Jörg J. Goronzy,Jennifer A. Maynard,Andrew D. Ellington,Gregory C. Ippolito,George Georgiou +19 more
TL;DR: It is discovered that the recently engineered xenopolymerase, RTX, is exceptionally resistant to cell lysate inhibition in single-cell emulsion droplets and capitalized on the characteristics of this enzyme to develop a simple, rapid, and inexpensive in-droplet overlap extension reverse transcription polymerase chain reaction method for NPS not requiring microfluidics or other specialized equipment.