Felix T. Wieland

TL;DR: It is found that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of theendosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide.

TL;DR: A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins and, with the exception of the V-ATPase, contains numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.

TL;DR: Eukaryotic life depends on the spatial and temporal organization of cellular membrane systems and general principles that underlie a broad variety of physiological processes, including cell surface growth, the biogenesis of distinct intracellular organelles, endocytosis, and the controlled release of hormones and neurotransmitters.

TL;DR: Findings suggest that VIP21/caveolin, through its cholesterol-binding properties, serves a specific function in microdomain formation during membrane trafficking in caveolae and apical transport vesicles.

TL;DR: The optimized ionization and fragmentation conditions described together with the principle of internal standardization by nonnatural analogues allow the rapid and quantitative determination of membrane lipid compositions down to sample amounts of 1000 cells.