Molecular Anatomy of a Trafficking Organelle
Shigeo Takamori,Matthew Holt,Katinka Stenius,Edward A. Lemke,Mads Grønborg,Dietmar Riedel,Henning Urlaub,Stephan Schenck,Britta Brügger,Philippe Ringler,Shirley A. Müller,Burkhard Rammner,Frauke Gräter,Jochen S. Hub,Bert L. de Groot,Gottfried Mieskes,Yoshinori Moriyama,Jürgen Klingauf,Helmut Grubmüller,John E. Heuser,Felix T. Wieland,Reinhard Jahn +21 more
TLDR
A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins and, with the exception of the V-ATPase, contains numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.About:
This article is published in Cell.The article was published on 2006-11-17 and is currently open access. It has received 2030 citations till now. The article focuses on the topics: Synaptic vesicle cycle & Vesicle-Associated Membrane Protein 2.read more
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Membrane lipids: where they are and how they behave.
TL;DR: How do cells apply anabolic and catabolic enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functions of their individual membranes?
Journal ArticleDOI
Molecular mechanism and physiological functions of clathrin-mediated endocytosis
TL;DR: Clathrin-mediated endocytosis is the endocytic portal into cells through which cargo is packaged into vesicles with the aid of a clathrin coat and is fundamental to neurotransmission, signal transduction and the regulation of many plasma membrane activities and is thus essential to higher eukaryotic life.
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Tau in physiology and pathology
Yipeng Wang,Eckhard Mandelkow +1 more
TL;DR: The expression, post-translational modifications and functions of tau in physiology and in pathophysiology are reviewed, including the identification of new physiological roles for t Tau in the brain.
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Neutrophil Extracellular Traps Contain Calprotectin, a Cytosolic Protein Complex Involved in Host Defense against Candida albicans
Constantin F. Urban,David Ermert,Monika Schmid,Ulrike Abu-Abed,Christian Goosmann,Wolfgang Nacken,Volker Brinkmann,Peter R. Jungblut,Arturo Zychlinsky +8 more
TL;DR: The present investigations confirmed the antifungal activity of calprotectin in vitro and demonstrated that it contributes to effective host defense against C. albicans in vivo.
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Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Christer S. Ejsing,Julio L. Sampaio,Vineeth Surendranath,Eva Duchoslav,Kim Ekroos,Robin W. Klemm,Kai Simons,Andrej Shevchenko +7 more
TL;DR: Comparative lipidomics demonstrated that growth temperature and defects in lipid biosynthesis induce ripple effects throughout the molecular composition of the yeast lipidome, establishing shotgun lipidomics as a powerful platform for complementing biochemical studies and other systems-level approaches.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
TL;DR: Silver staining allows a substantial shortening of sample preparation time and may, therefore, be preferable over Coomassie staining, and this work removes a major obstacle to the low-level sequence analysis of proteins separated on polyacrylamide gels.
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A simplification of the protein assay method of Lowry et al. which is more generally applicable
TL;DR: A simple method based on a linear log-log protein standard curve is presented to permit rapid and totally objective protein analysis using small programmable calculators.