Grigory Tsaur

TL;DR: Long-distance inverse-polymerase chain reaction was used to characterize the chromosomal rearrangement of individual acute leukemia patients and revealed a total of 121 different MLL rearrangements, of which 79 TPGs are now characterized at the molecular level.

TL;DR: In this paper, the distribution of MLL breakpoints for clinical subtypes (acute lymphoblastic leukemia, acute myeloid leukemia, pediatric and adult) and fused translocation partner genes (TPGs) were presented, including novel MLL fusion genes.

TL;DR: The UKALL-CNA classifier is a robust prognostic tool that can be deployed in different trial settings and used to refine established cytogenetic risk groups.

TL;DR: The data do not support the concept of re-stratification and modification of therapy based on qualitative CSF investigation by MFC, however, MFC is a highly sensitive technique ofCSF investigation improving the definition of CNS involvement in childhood ALL, and quantitative measurement of blast cells in CSF, if well-organized, can be a useful additional tool for stratification of patients in clinical trials.

TL;DR: The presented cases showed consistency of gene rearrangements and fusion transcripts across initially diagnosed leukemia and lineage switch, and in two of six patients, the clonal architecture assessed by IG/TR gene rearranged was stable, while in others, loss of clones or gain of new clones was noted.