Mykhailo Girych

TL;DR: In this paper, the transmembrane domain of tyrosine kinase receptor 2 (TRKB), the brain-derived neurotrophic factor (BDNF) receptor that promotes neuronal plasticity and antidepressant responses, has a cholesterol-sensing function that mediates synaptic effects of cholesterol.

TL;DR: It is shown that by interpreting changes in the choline headgroup order parameters according to the 'molecular electrometer' concept, one can directly compare the ion binding affinities between simulations and experiments, and strongly support the view that in contrast to Ca2+ and other multivalent ions, Na- and other monovalent ions do not specifically bind to phosphatidylcholine lipid bilayers at sub-molar concentrations.

TL;DR: A novel combined ThT-CR fluorescence assay for amyloid identification is designed, which not only strengthens the reliability of the ThT assay, but also provides new opportunities for structural characterization of amyloids fibrils.

TL;DR: Two possible mechanisms by which cyanines can inhibit the insulin fibrillization are highlighted: i) stabilization of the native protein structure followed by the retardation of the protein nucleation (all dyes); and ii) blocking the lateral extension of β-sheets via the dye-protein stacking interactions (3-3, 3-5, 5-3 and 5-9).

TL;DR: Förster resonance energy transfer between tryptophan as a donor and Thioflavin T as an acceptor was employed to obtain structural information on the amyloid fibrils formed by apoA-I variant 1-83/G26R/W@8 to provide evidence for the presence of two types of ThT binding sites with similar stoichiometries.