Institution
BIA Separations (Slovenia)
Company•Ljubljana, Slovenia•
About: BIA Separations (Slovenia) is a company organization based out in Ljubljana, Slovenia. It is known for research contribution in the topics: Monolithic HPLC column & Monolith. The organization has 84 authors who have published 152 publications receiving 4883 citations.
Papers published on a yearly basis
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TL;DR: Efficient chromatographic purification of tomato mosaic virus from plant material is described and the purification process was shortened from 5 days to 2 hours, demonstrating the potential of short monolithic column technology for purification and analysis of different viruses.
67 citations
TL;DR: In this article, the isocratic separation of oligonucleotides in the ion exchange mode on thin GMA-EDMA monoliths in the form of commercially available CIM (Convective interaction media) disks is presented.
66 citations
TL;DR: The kinetics of immobilization was studied in detail for trypsin under dynamic conditions and revealed that the enzyme immobilized via imidazole carbamate groups already reached its highest activity in 5 min, in contrast, a much longer time was required for immobilization via epoxy groups.
Abstract: Monolithic Convective Interaction Media (CIM) have been activated with epoxide and imidazole carbamate functionalities and used as supports for covalent immobilization of protein A, deoxyribonuclease I, and trypsin. The efficiency of immobilization for these proteins was determined from the amount of bound IgG, degradation of DNA, and hydrolysis of Nalpha-benzoyl-L-arginine ethyl ester, respectively. The respective biological activities of trypsin and the binding capacity of protein A immobilized via imidazole carbamate groups were 11.45 and 2.25 times higher than those obtained for epoxide matrix while they were practically equal for deoxyribonuclease I. The kinetics of immobilization was studied in detail for trypsin under dynamic conditions and revealed that the enzyme immobilized via imidazole carbamate groups already reached its highest activity in 5 min. In contrast, a much longer time was required for immobilization via epoxy groups.
66 citations
TL;DR: A pDNA purification process designed on two different CIM monolithic columns, based on anion-exchange (AEX) chromatography and hydrophobic interaction chromatography (HIC) chemistry, found to be reproducible, scalable, and exhibits high productivity.
64 citations
TL;DR: In this article, the enrichment of virus RNA on short monolithic columns prior to molecular detection of viruses is described, and the results show that it is possible to bind viral RNA on monoliths and concentrate viral nucleic acids from a fairly dilute sample.
63 citations
Authors
Showing all 84 results
| Name | H-index | Papers | Citations |
|---|---|---|---|
| Aleš Štrancar | 38 | 122 | 3748 |
| Djuro Josic | 36 | 177 | 4580 |
| Aleš Podgornik | 36 | 90 | 3274 |
| Thomas Muster | 33 | 67 | 7766 |
| Miloš Barut | 23 | 45 | 1500 |
| Matjaž Peterka | 17 | 32 | 1222 |
| Petra Kramberger | 14 | 23 | 642 |
| Janez Jančar | 11 | 14 | 425 |
| Jana Vidič | 10 | 19 | 686 |
| Nika Lendero Krajnc | 10 | 19 | 395 |
| Urh Černigoj | 9 | 25 | 267 |
| Rok Košir | 9 | 14 | 456 |
| Peter Brne | 8 | 8 | 330 |
| Marko Banjac | 7 | 10 | 206 |
| Lidija Urbas | 7 | 8 | 241 |