Institution
BIA Separations (Slovenia)
Company•Ljubljana, Slovenia•
About: BIA Separations (Slovenia) is a company organization based out in Ljubljana, Slovenia. It is known for research contribution in the topics: Monolithic HPLC column & Monolith. The organization has 84 authors who have published 152 publications receiving 4883 citations.
Papers published on a yearly basis
Papers
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TL;DR: To examine certain process conditions for purification of large plasmids with the size of up to 93 kb, cell lysis and storage conditions were investigated and the impact of chromatographic system and methacrylate monolithic column on the degradation of plasmid molecules under nonbinding conditions at different flow rates was studied.
Abstract: The rapid evolution of gene therapy and DNA vaccines results in an increasing interest in producing large quantities of pharmaceutical grade plasmid DNA. Most current clinical trials involve plasmids of 10 kb or smaller in size, however, future requirements for multigene vectors including extensive control regions may require the production of larger plasmids, e. g., 20 kb and bigger. The objective of this study was to examine certain process conditions for purification of large plasmids with the size of up to 93 kb. Since there is a lack of knowledge about production and purification of bigger plasmid DNA, cell lysis and storage conditions were investigated. The impact of chromatographic system and methacrylate monolithic column on the degradation of plasmid molecules under nonbinding conditions at different flow rates was studied. Furthermore, capacity measurements varying salt concentration in loading buffer were performed and the capacities up to 13 mg of plasmid per mL of the monolithic column were obtained. The capacity flow independence in the range from 130 to 370 cm/h was observed. Using high resolution monolithic column the separation of linear and supercoiled isoforms of large plasmids was obtained. Last but not least, since the baseline separation of RNA and pDNA was achieved, the one step purification on larger CIM DEAE 8 mL tube monolithic column was performed and the fractions were analyzed by CIM analytical monolithic columns.
36 citations
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TL;DR: The selected operating conditions using quaternary amine monolithic supports enabled highly efficient binding, purification and concentration of rubella virus from complex biological suspension without additional procedures, indicating the possibility of using monoliths to improve the rubellairus yields in productions where high virus titers during cultivation can hardly be achieved.
34 citations
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TL;DR: A novel monolithic chromatographic support bearing multimodal histamine (HISA) groups was developed and characterized, enabling separation of supercoiled (sc) pDNA from other plasmid isoforms (and other process related impurities) by adjusting salt or pH gradient.
34 citations
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TL;DR: In this article, the physicochemical properties of histamine ligand in a supercoiled plasmid DNA purification process from an Escherichia coli clarified lysate, where the emphasis is given to the elution strategy that allows higher selectivity and efficient removal of other impurities besides the open circular isoform.
34 citations
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TL;DR: This method can be successfully applied not only to an in-process control of the purification of factor IX but also to other biopolymers from human plasma.
33 citations
Authors
Showing all 84 results
Name | H-index | Papers | Citations |
---|---|---|---|
Aleš Štrancar | 38 | 122 | 3748 |
Djuro Josic | 36 | 177 | 4580 |
Aleš Podgornik | 36 | 90 | 3274 |
Thomas Muster | 33 | 67 | 7766 |
Miloš Barut | 23 | 45 | 1500 |
Matjaž Peterka | 17 | 32 | 1222 |
Petra Kramberger | 14 | 23 | 642 |
Janez Jančar | 11 | 14 | 425 |
Jana Vidič | 10 | 19 | 686 |
Nika Lendero Krajnc | 10 | 19 | 395 |
Urh Černigoj | 9 | 25 | 267 |
Rok Košir | 9 | 14 | 456 |
Peter Brne | 8 | 8 | 330 |
Marko Banjac | 7 | 10 | 206 |
Lidija Urbas | 7 | 8 | 241 |