Institution
BIA Separations (Slovenia)
Company•Ljubljana, Slovenia•
About: BIA Separations (Slovenia) is a company organization based out in Ljubljana, Slovenia. It is known for research contribution in the topics: Monolithic HPLC column & Monolith. The organization has 84 authors who have published 152 publications receiving 4883 citations.
Papers published on a yearly basis
Papers
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TL;DR: A unique and simple diagnostic scheme for rapid, efficient, and sensitive monitoring of irrigation waters that could also be adopted for other plant, human or animal viruses is proposed.
Abstract: A quantitative RT real-time PCR method was developed for the detection and quantification of Tomato mosaic virus (ToMV) in irrigation waters. These have rarely been monitored for the presence of plant pathogenic viruses, mostly due to the lack of efficient and sensitive detection methods. The newly developed method presented here offers a novel approach in monitoring the health status of environmental waters. ToMV was reliably detected at as low as 12 viral particles per real-time PCR reaction, which corresponds to the initial concentration of approximately 4.2 × 10−10 mg (6,300 viral particles) of ToMV per ml of sample. The sensitivity of the method was further improved by including the Convective Interaction Media® (CIM) monolithic chromatographic columns for quick and efficient concentration of original water samples. Seven out of nine water sources from different locations in Slovenia tested positive for ToMV, after concentrating the sample. Four samples tested ToMV-positive without the concentrating procedure. The presence and integrity of infective ToMV particles in the original sample, as well as in the chromatographic fraction, was confirmed using different methods from test plants, DAS ELISA to electron microscopy and real-time PCR. In this study, we propose a unique and simple diagnostic scheme for rapid, efficient, and sensitive monitoring of irrigation waters that could also be adopted for other plant, human or animal viruses.
45 citations
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TL;DR: Surprisingly, it was found that the critical part of the glass surface modification procedure is glass pre-treatment, and the highest values were found for soda-lime glass.
43 citations
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TL;DR: The duration of pH transient was shown to be linearly proportional to the total ionic capacity and was used to estimate protein dynamic binding capacity of the resin and to distinguish between cation and anion or weak and strong ion exchange chromatographic supports.
43 citations
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TL;DR: The method permits the quantification of albumin and transferrin in IgG concentrates and has been successfully validated.
41 citations
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TL;DR: A chromatographic process based on monoliths for purification of infective baculovirus without prior concentration step has been established and recovery of infectious virus was highly influenced by composition and age of supernatant.
41 citations
Authors
Showing all 84 results
Name | H-index | Papers | Citations |
---|---|---|---|
Aleš Štrancar | 38 | 122 | 3748 |
Djuro Josic | 36 | 177 | 4580 |
Aleš Podgornik | 36 | 90 | 3274 |
Thomas Muster | 33 | 67 | 7766 |
Miloš Barut | 23 | 45 | 1500 |
Matjaž Peterka | 17 | 32 | 1222 |
Petra Kramberger | 14 | 23 | 642 |
Janez Jančar | 11 | 14 | 425 |
Jana Vidič | 10 | 19 | 686 |
Nika Lendero Krajnc | 10 | 19 | 395 |
Urh Černigoj | 9 | 25 | 267 |
Rok Košir | 9 | 14 | 456 |
Peter Brne | 8 | 8 | 330 |
Marko Banjac | 7 | 10 | 206 |
Lidija Urbas | 7 | 8 | 241 |