Central Agricultural University

About: Central Agricultural University is a education organization based out in Imphal, Manipur, India. It is known for research contribution in the topics: Population & Agriculture. The organization has 1116 authors who have published 1157 publications receiving 9217 citations.

Association mapping of genomic loci linked with Fusarium wilt resistance (Foc2) in chickpea

Abstract: Improving plant resistance against Fusarium wilt (FW) is key to sustaining chickpea production worldwide. Given this, the current study tested a set of 75 FW-responsive chickpea breeding lines including checks in a wilt-sick plot for two consecutive years (2016 and 2017). Genetic diversity analysis using 75 simple sequence repeats (SSRs) revealed a total of 267 alleles with an average of 3.56 alleles per marker. The entire set was divided into two major classes based on clustering method and factorial analysis. Similarly, STRUCTURE analysis placed the 75 genotypes into three distinct sub-groups (K = 3). Marker-trait association (MTA) analysis using the generalized linear model approach revealed nine and eight significant MTAs for FW resistance in the years 2016 and 2017, respectively. Three significant MTAs were obtained for FW resistance following the mixed linear model approach for both years. The SSR markers CESSR433, NCPGR21 and ICCM0284 could be potentially employed for targeted and accelerated improvement of FW resistance in chickpea. To the best of our knowledge, this is the first report on association mapping of the genomic loci controlling FW (Foc2) resistance in chickpea.

Seedling root-dip in phosphorus and biofertilizer added soil slurry method of nutrient management for transplanted rice in acid soil

Abstract: Management of phosphorus (P) in acid soils is becoming more challenging in the anticipated scenario of potential phosphate crisis in agriculture, because the P-use and -recovery efficiencies (PUE and PRE) of existing P management methods are notoriously low in acid soils. This investigation reported a rhizosphere-based P management method for improving P nutrition of rice (Oryza sativa L.) seedlings at the time of transplantation in order to support better root growth on post transplantation. By performing two independent incubation experiments, the critical doses of orthophosphate and incubation duration for seedling root-dip (SRD) in single super phosphate (SSP) amended soil slurry (a sandy clay loam Inceptisol, pH 4.3) were found to be 112.5 mg P kg-1 soil and 10 h, respectively using the critical curve approach. In field experiments, the rhizosphere-based P method (SRD in soil slurry + phosphate solubilizing bacteria, PSB + rock phosphate, RP (30 kg P2O5 ha -1) performed better than SSP broadcast as basal application (60 kg P2O5 ha -1) in terms of more root volume and P uptake of shoot and root at 45 days after transplantation (DAT), higher P uptake and content in rice grain and straw, enhanced PUE and PRE, comparable grain yield and 50% reduction in P fertilizer input quantity. This rhizosphere based P management (SRD in soil slurry+PSB+RP) method may be vigorously exploited for managing P nutrition in transplanted rice grown in acid soils.

Dietary supplementation of L-tryptophan: Effect on growth and survival of Pabda, Ompok bimaculatus (Bloch) fry

Abstract: A 30-day experiment was conducted to assess the effect of tryptophan-supplemented diets on growth and survival of Pabda, Ompok bimaculatus fry. Fifty pabda fry (0.114 ± 0.02 g; 2.45 ± 0.13 ...

Characterization of Multidrug-Resistant Biofilm-Producing Escherichia coli and Klebsiella pneumoniae in Healthy Cattle and Cattle with Diarrhea.

Abstract: This study describes comparative occurrence and characterization of multidrug-resistant (MDR) Escherichia coli and Klebsiella pneumoniae (KP) in healthy cattle (HC) and cattle with diarrhea (DC) in India. During 2018-2020, 72 MDR isolates, including 35 E. coli (DC: 27; HC 8) and 37 K. pneumoniae (DC: 34; HC: 3), from 251 rectal swabs (DC: 219; HC: 32) were investigated for extended-spectrum beta-lactamase (ESBL), AmpC type β-lactamase and carbapenemase production, antimicrobial susceptibility profile, biofilm production, and efflux pump activity. Fifty-five MDR isolates were ESBL producers (ESBLPs) (DC: 50; HC: 5) and ESBLPs from DC were coresistant to multiple antibiotics. The blaCTX-M gene (50) was the most frequently detected β-lactamases followed by blaAmpC (22), blaTEM1 (13), blaCMY-6 (6), blaOXA1 (5), blaPER (2), blaDHA, and blaFOX and blaSHV12 (1 each). Plasmid-mediated quinolone resistance determinants qnrB, qnrS, qnrA, and qepA were detected in 18, 16, 2, and 3 isolates, respectively. Twenty three isolates revealed mutation in gyrA and parC genes. Tetracycline-resistance markers tetA, tetB, tetC, and tetE were detected in 33, 10, 3, and 2 isolates, respectively. Only one of the 41 imipenem-resistant isolates harbored blaNDM-5 and two were colistin-resistant. Altogether, 20 MDR isolates were strong biofilm producers and 19 harbored different virulence factors. This is the first ever report from India on the presence of MDR Enterobacteriaceae with resistance to even last-resort antimicrobials in the bovine diarrhea.