China University of Petroleum

About: China University of Petroleum is a education organization based out in Beijing, China. It is known for research contribution in the topics: Catalysis & Oil shale. The organization has 39802 authors who have published 39151 publications receiving 483760 citations. The organization is also known as: Zhōngguó Shíyóu Dàxué & China University of Petroleum (Beijing).

Single-Molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53

Abstract: The state of oligomerization of the tumor suppressor p53 is an important factor in its various biological functions. It has a well-defined tetramerization domain, and the protein exists as monomers, dimers and tetramers in equilibrium. The dissociation constants between oligomeric forms are so low that they are at the limits of measurement by conventional methods in vitro. Here, we have used the high sensitivity of single-molecule methods to measure the equilibria and kinetics of oligomerization of full-length p53 and its isolated tetramerization domain, p53tet, at physiological temperature, pH and ionic strength using fluorescence correlation spectroscopy (FCS) in vitro. The dissociation constant at 37 °C for tetramers dissociating into dimers for full-length p53 was 50 ± 7 nM, and the corresponding value for dimers into monomers was 0.55 ± 0.08 nM. The half-lives for the two processes were 20 and 50 min, respectively. The equivalent quantities for p53tet were 150 ± 10 nM, 1.0 ± 0.14 nM, 2.5 ± 0.4 min and 13 ± 2 min. The data suggest that unligated p53 in unstressed cells should be predominantly dimeric. Single-molecule FCS is a useful procedure for measuring dissociation equilibria, kinetics and aggregation at extreme sensitivity.

CAIS: A Copy Adjustable Incentive Scheme in Community-Based Socially Aware Networking

Abstract: Socially aware networking (SAN) is a new communication paradigm, in which the social characteristics of mobile nodes are exploited to improve the performance of data distribution. In SAN, mobile carriers may exhibit selfish behaviors and refuse to relay messages for others for various reasons, such as limited resources (e.g., buffer, energy, and bandwidth) or social relationships. Several incentive schemes have recently been investigated to stimulate selfish users for cooperation in data forwarding. However, a majority of the existing methods have not fully studied nodes' social relationships in their selfish behaviors. In this paper, we propose a copy adjustable incentive scheme (CAIS), which adopts the virtual credit concept to stimulate selfish nodes to cooperate in data forwarding. In CAIS, we consider a network in which the nodes are divided into certain communities based on their social relationships. Then, we apply two types of credits, i.e., social credit and nonsocial credit, to reward the nodes when they relay data for other nodes inside their community or outsiders, respectively. Based on our mechanism, the number of messages a node can replicate to other nodes is adjusted according to its cooperation level and earned credits. To further improve the performance of CAIS, a single-copy data replication policy is employed, which manages the credit distribution of each node according to its available resources. The results of our extensive experiments using both synthetic and trace-driven simulations illustrate that CAIS copes well with node selfishness in community-based networks and outperforms other benchmark protocols with high data delivery ratio, low communication overhead, and short data delivery latency.