Institution
Duquesne University
Education•Pittsburgh, Pennsylvania, United States•
About: Duquesne University is a education organization based out in Pittsburgh, Pennsylvania, United States. It is known for research contribution in the topics: Population & Health care. The organization has 3615 authors who have published 7169 publications receiving 180066 citations. The organization is also known as: Duquesne University of the Holy Spirit.
Papers published on a yearly basis
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Shanghai Jiao Tong University1, Northwestern University2, University of California, San Francisco3, Duke University4, University of Southern California5, Saitama Medical University6, Kyorin University7, Duquesne University8, University of Pittsburgh9, Ohio State University10, University of California, San Diego11
TL;DR: It is determined that the discoidina neuropilin-like membrane protein DCBLD2 is upregulated in clinical specimens of glioblastomas and head and neck cancers and is required for EGFR-stimulated tumorigenesis and suggested DCBLd2 and TRAF6 as potential therapeutic targets for human cancers that are associated with EGFR activation.
Abstract: Aberrant activation of EGFR in human cancers promotes tumorigenesis through stimulation of AKT signaling. Here, we determined that the discoidina neuropilin-like membrane protein DCBLD2 is upregulated in clinical specimens of glioblastomas and head and neck cancers (HNCs) and is required for EGFR-stimulated tumorigenesis. In multiple cancer cell lines, EGFR activated phosphorylation of tyrosine 750 (Y750) of DCBLD2, which is located within a recently identified binding motif for TNF receptor-associated factor 6 (TRAF6). Consequently, phosphorylation of DCBLD2 Y750 recruited TRAF6, leading to increased TRAF6 E3 ubiquitin ligase activity and subsequent activation of AKT, thereby enhancing EGFR-driven tumorigenesis. Moreover, evaluation of patient samples of gliomas and HNCs revealed an association among EGFR activation, DCBLD2 phosphorylation, and poor prognoses. Together, our findings uncover a pathway in which DCBLD2 functions as a signal relay for oncogenic EGFR signaling to promote tumorigenesis and suggest DCBLD2 and TRAF6 as potential therapeutic targets for human cancers that are associated with EGFR activation.
73 citations
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TL;DR: Glycerophosphocholine is formed via the deacylation of the phospholipid phosphatidylcholine, and glycerophospho[3H]choline supplied in the growth medium accumulates to a much greater extent in the intracellular fraction of a YPL110Δ strain than in a wild type strain.
73 citations
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TL;DR: PFC nanoemulsions enabling real-time optical detection of intracellular pH measurements in living cells in response to pharmacological manipulations allow the study of the fate of the PFC tracer inside the labeled cell, which is important for understanding the P FC cell loading dynamics, nanoemulsion stability and cell viability over time.
Abstract: We report the synthesis and formulation of unique perfluorocarbon (PFC) nanoemulsions enabling intracellular pH measurements in living cells via fluorescent microscopy and flow cytometry. These nanoemulsions are formulated to readily enter cells upon coincubation and contain two cyanine-based fluorescent reporters covalently bound to the PFC molecules, specifically Cy3-PFC and CypHer5-PFC conjugates. The spectral and pH-sensing properties of the nanoemulsions were characterized in vitro and showed the unaltered spectral behavior of dyes after formulation. In rat 9L glioma cells loaded with nanoemulsion, the local pH of nanoemulsions was longitudinally quantified using optical microscopy and flow cytometry and displayed a steady decrease in pH to a level of 5.5 over 3 h, indicating rapid uptake of nanoemulsion to acidic compartments. Overall, these reagents enable real-time optical detection of intracellular pH in living cells in response to pharmacological manipulations. Moreover, recent approaches for in...
73 citations
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TL;DR: Assessing sedative/pain/paralytic drug use patterns including dosage, route, selection, and combination therapy by collecting actual drug administration data from multiple centers suggested a preference for opiate and benzodiazepine therapy.
Abstract: ObjectiveTo solicit practitioner-perceived opinions regarding current sedative/analgesic/paralytic practice including drug selection, admixture methods, and methods of assessing patient response to...
73 citations
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TL;DR: It is proposed that to isolate elements functionally, only minor changes might be necessary between elements, in both inverted terminal repeat and amino acid sequence, and a mechanism to explain mariner diversification based on this phenomenon is proposed.
Abstract: Mariners are a large family of eukaryotic DNA-mediated transposable elements that move via a cut-and-paste mechanism. Several features of the evolutionary history of mariners are unusual. First, they appear to undergo horizontal transfer commonly between species on an evolutionary timescale. They can do this because they are able to transpose using only their own self-encoded transposase and not host-specific factors. One consequence of this phenomenon is that more than one kind of mariner can be present in the same genome. We hypothesized that two mariners occupying the same genome would not interact. We tested the limits of mariner interactions using an in vitro transposition system, purified mariner transposases, and DNAse I footprinting. Only mariner elements that were very closely related to each other (ca. 84% identity) cross-mobilized, and then inefficiently. Because of the dramatic suppression of transposition between closely related elements, we propose that to isolate elements functionally, only minor changes might be necessary between elements, in both inverted terminal repeat and amino acid sequence. We further propose a mechanism to explain mariner diversification based on this phenomenon.
73 citations
Authors
Showing all 3668 results
Name | H-index | Papers | Citations |
---|---|---|---|
Krzysztof Matyjaszewski | 169 | 1431 | 128585 |
William L. Jorgensen | 108 | 586 | 95112 |
John C. Avise | 105 | 413 | 53088 |
Rongchao Jin | 101 | 332 | 42920 |
Paul Knochel | 99 | 2373 | 44786 |
Gwendolen Jull | 87 | 410 | 26556 |
Hugh M. Robertson | 83 | 197 | 27173 |
Peter Wipf | 83 | 767 | 25316 |
Ivet Bahar | 78 | 391 | 24228 |
Luk N. Van Wassenhove | 78 | 322 | 29163 |
Carl H. Snyderman | 76 | 481 | 22390 |
Ronald S. Oremland | 76 | 198 | 19671 |
Jeffrey L. Brodsky | 71 | 256 | 18315 |
Maarten J. Postma | 62 | 753 | 33409 |
Alan J. Russell | 62 | 280 | 13894 |