Showing papers by "Nanchang University published in 2012"

Observation of Microscale Superlubricity in Graphite

Abstract: Upon shearing a microscale lithographically defined graphite mesa, the sheared section retracts spontaneously to minimize interface energy. Here, we demonstrate a sixfold symmetry of the self-retraction and provide a first experimental estimate of the frictional force involved, as direct evidence that the self-retraction is due to superlubricity, where ultralow friction occurs between incommensurate surfaces. The effect is remarkable because it occurs reproducibly under ambient conditions and over a contact area of up to 10×10 μm2, more than 7 orders of magnitude larger than previous scanning-probe-based studies of superlubricity in graphite. By analyzing the sheared interface, we show how the grain structure of highly oriented pyrolitic graphite determines the probability of self-retraction. Our results demonstrate that such self-retraction provides a novel probe of superlubricity, and the robustness of the phenomenon opens the way for practical applications of superlubricity in micromechanical systems.

Role of reactive oxygen species in the antibacterial mechanism of silver nanoparticles on Escherichia coli O157:H7.

Abstract: In this study, the conditions and mechanism of antibacterial activity of hydrophilic polymer coated silver nanoparticles (AgNPs) against E. coli O157:H7 (CMCC44828) as model pathogen was studied. The AgNPs were coated with amphiphilic polymer that introduced carboxyl groups on the surface to make it water-soluble. The AgNPs were exposed to various treatment conditions of pH and temperature before these were combined with the E. coli. The mechanism of the antibacterial activity was studied through the formation of reactive oxygen species (ROS) that was later suppressed with antioxidant to establish correlation with the AgNPs antimicrobial activity. Studies were carried out at both anaerobic and aerobic conditions. The results indicated that 5 mg/L AgNPs inhibited ~50% of the growth of 106 colony forming units per milliliter (cfu/mL) E. coli cells in liquid Luria–Bertani (LB) medium. This dose-dependent antimicrobial activity was higher at increased temperature (37°C) but was lower when the AgNPs were treated with acid at pH 2 before exposure to the bacteria. It was also established that the conditions of higher antimicrobial effect generated more ROS that was dependent on the presence of oxygen. The antibacterial activity was suppressed in the presence of an antioxidant.

Novel Fungal Pelletization-Assisted Technology for Algae Harvesting and Wastewater Treatment

Abstract: A novel fungi pelletization-assisted bioflocculation technology was developed for efficient algae harvesting and wastewater treatment. Microalga Chlorella vulgaris UMN235 and two locally isolated fungal species Aspergillus sp. UMN F01 and UMN F02 were used to study the effect of various cultural conditions on pelletization process for fungi–algae complex. The results showed that pH was the key factor affecting formation of fungi–algae pellet, and pH could be controlled by adjusting glucose concentration and fungal spore number added. The best pelletization happened when adding 20 g/L glucose and approximately 1.2E8/L spores in BG-11 medium, under which almost 100 % of algal cells were captured onto the pellets with shorter retention time. The fungi–algae pellets can be easily harvested by simple filtration due to its large size (2–5 mm). The filtered fungi–algae pellets were reused as immobilized cells for treatment wastewaters and the nutrient removal rates of 100, 58.85, 89.83, and 62.53 % (for centrate) and 23.23, 44.68, 84.70, and 70.34 % (for diluted swine manure wastewater) for ammonium, total nitrogen, total phosphorus, and chemical oxygen demand, respectively, under both 1- and 2-day cultivations. The novel technology developed is highly promising compared with current algae harvesting and biological wastewater treatment technologies in the literature.

Interlayer binding energy of graphite: A mesoscopic determination from deformation

Abstract: Despite the interlayer binding energy being one of the most important material properties of graphite, direct experimental determination of this property is yet to be reported In this paper, we present an experimental method to directly measure the interlayer binding energy of highly oriented pyrolytic graphite (HOPG) The obtained value of the binding energy is 019 ($\ifmmode\pm\else\textpm\fi{}$001) J/m${}^{2}$, which can serve as a benchmark for other theoretical and experimental works related to graphite/graphene systems

Probing the binding of the flavonoid diosmetin to human serum albumin by multispectroscopic techniques.

Abstract: The binding mechanism of molecular interaction between diosmetin and human serum albumin (HSA) in a pH 7.4 phosphate buffer was studied using atomic force microscopy (AFM) and various spectroscopic techniques including fluorescence, resonance light scattering (RLS), UV–vis absorption, circular dichroism (CD), and Fourier transform infrared (FT–IR) spectroscopy. Fluorescence data revealed that the fluorescence quenching of HSA by diosmetin was a static quenching procedure. The binding constants and number of binding sites were evaluated at different temperatures. The RLS spectra and AFM images showed that the dimension of the individual HSA molecules were larger after interaction with diosmetin. The thermodynamic parameters, ΔH° and ΔS° were calculated to be −24.56 kJ mol–1 and 14.67 J mol–1 K–1, respectively, suggesting that the binding of diosmtin to HSA was driven mainly by hydrophobic interactions and hydrogen bonds. The displacement studies and denaturation experiments in the presence of urea indicate...

miR-210 activates notch signaling pathway in angiogenesis induced by cerebral ischemia.

Abstract: The compensatory angiogenesis that occurs after cerebral ischemia increases blood flow to the injured area and limits extension of the ischemic penumbra. In this way, it improves the local blood supply. Fostering compensatory angiogenesis is an effective treatment for ischemic cerebrovascular disease. However, angiogenesis in the adult organism is a complex, multi-step process, and the mechanisms underlying the regulation of angiogenesis are not well understood. Although Notch signaling reportedly regulates the vascularization process that occurs in ischemic tissues, little is known about the role of Notch signaling in the regulation of ischemia-induced angiogenesis after ischemic stroke. Recent research has indicated that miR-210, a hypoxia-induced microRNA, plays a crucial role in regulating the biological processes that occur in blood vessel endothelial cells under hypoxic conditions. This study was undertaken to investigate the role of miR-210 in regulating angiogenesis in response to brain ischemia injury and the role of the Notch pathway in the body's response. We found miR-210 to be significantly up-regulated in adult rat ischemic brain cortexes in which the expression of Notch1 signaling molecules was also increased. Hypoxic models of human umbilical vein endothelial cells (HUVE-12) were used to assess changes in miR-210 and Notch1 expression in endothelial cells. Results were consistent with in vivo findings. To determine the molecular mechanisms behind these phenomena, we transfected HUVE-12 cells with miR-210 recombinant lentiviral vectors. We found that miR-210 overexpression caused up-regulation of Notch1 signaling molecules and induced endothelial cells to migrate and form capillary-like structures on Matrigel. These data suggest that miR-210 is involved in the regulation of angiogenesis in response to ischemic injury to the brain. Up-regulation of miR-210 can activate the Notch signaling pathway, which may contribute to angiogenesis after cerebral ischemia.

Genome-wide association study in Chinese men identifies two new prostate cancer risk loci at 9q31.2 and 19q13.4

Abstract: Yinghao Sun and colleagues report a genome-wide association study for prostate cancer in Han Chinese men. They identify two new risk-associated loci at chromosomes 9q31 and 19q13. Prostate cancer risk–associated variants have been reported in populations of European descent, African-Americans and Japanese using genome-wide association studies (GWAS). To systematically investigate prostate cancer risk–associated variants in Chinese men, we performed the first GWAS in Han Chinese. In addition to confirming several associations reported in other ancestry groups, this study identified two new risk-associated loci for prostate cancer on chromosomes 9q31.2 (rs817826, P = 5.45 × 10−14) and 19q13.4 (rs103294, P = 5.34 × 10−16) in 4,484 prostate cancer cases and 8,934 controls. The rs103294 marker at 19q13.4 is in strong linkage equilibrium with a 6.7-kb germline deletion that removes the first six of seven exons in LILRA3, a gene regulating inflammatory response, and was significantly associated with the mRNA expression of LILRA3 in T cells (P < 1 × 10−4). These findings may advance the understanding of genetic susceptibility to prostate cancer.

Kinetic structure and wave properties associated with sharp dipolarization front observed by Cluster

Abstract: Multiple dipolarization fronts (DFs) were observed by Cluster spacecraft in the magnetotail during a substorm. These DFs were kinetic structures, embedded in the bursty plasma flow, and moved earthward (mainly) and dawnward. Intense electric field, parallel and perpendicular currents were detected in the DF layer. These front layers were energy dissipation region (load region) where the energy of electromagnetic fields were transferred to the plasma thermal and kinetic energy. This dissipation was dominated by electrons. There were enhancements of plasma waves around the DF region: wavelet results show that wave activities around the ion cyclotron frequency in the front layer were generated by Alfven ion cyclotron instability; whistler waves were also detected before, during and after the DFs, which are triggered by electron temperature anisotropy and coincident with enhancement of energetic electron fluxes. The observation of these waves could be important for the understanding of evolution of DF and electron energization during the substorm. We discuss the generation mechanism of the DFs and suggest that these DFs were generated in the process of transient reconnection, and then traveled toward the Earth.

Upregulation of microRNA-210 regulates renal angiogenesis mediated by activation of VEGF signaling pathway under ischemia/perfusion injury in vivo and in vitro.

Abstract: Background: MicroRNAs (miRNAs) are endogenous, non-coding, small RNAs that regulate gene expression and function, but little is known about regulation of miRNAs in the kidneys under normal or pathologic conditions. Here, we sought to investigate the potential involvement of miRNAs in renal ischemia/reperfusion (I/R) injury and angiogenesis and to define some of the miRNAs possibly associated with renal angiogenesis. Methods and Results: Male Balb/c mice were subjected to a standard renal I/R. CD31 immunostaining indicated a significant increase of microvessels in the ischemic region. VEGF and VEGFR2 expression were increased in renal I/R at both the mRNA and protein levels which were detected by qRT-PCR and Western blot, respectively. More importantly, 76 microRNAs exhibited more than 2-fold changes using Agilent microRNA microarray, which contains downregulation of 40 miRNAs and upregulation of 36 miRNAs. Upregulation of miR-210 was confirmed by qRT-PCR with prominent changes at 4 and 24 h after reperfusion. Furthermore, overexpression of miR-210 in HUVEC-12 cells enhances VEGF and VEGFR2 expression and promotes angiogenesis on Matrigel in vitro. Conclusion: These findings suggest miR-210 may be involved in targeting the VEGF signaling pathway to regulate angiogenesis after renal I/R injury, which provides novel insights into the angiogenesis mechanism of renal I/R injury.

How to interpret epicardial adipose tissue as a cause of coronary artery disease: a meta-analysis

Abstract: BACKGROUND Experimental and clinical studies have suggested that epicardial adipose tissue (EAT) may cause coronary artery disease (CAD). A meta-analysis was conducted to investigate the relationship between EAT and CAD. METHODS A systematic literature search of Cochrane, Medline, Pubmed, Elsevier, Springerlink, Ovid, and Embase from their respective inceptions to August 2011 was conducted using specific search terms such as 'epicardial adipose tissue' and 'epicardial fat'. Data were extracted from applicable articles and mean differences or risk ratio, including 95% confidence intervals (CI), were calculated using RevMan 5.1 software. RESULTS A total of 15 case-control studies and one case-sectional study (N=2872 patients) were identified. Compared with the non-CAD group, EAT thickness and volume were significantly higher in the CAD group (mean difference 1.57 mm, 95% CI: 0.74, 2.40, P<0.00001; mean difference 15.22 ml, 95% CI: 7.58, 22.87, P<0.0001). Patients in the higher EAT tertile (≥ 100 ml) were more likely to have CAD compared with those in the lower EAT tertile (<100 ml) (risk ratio 0.69, 95% CI: 0.52, 0.92, P=0.01). Patients with coronary plaque also had increased EAT volume compared with patients without coronary plaque (mean difference 24.90 ml, 95% CI: 9.99, 39.81 P=0.001). EAT volume was not different in patients whose coronary artery calcium scores were less than or equal to 10 or greater than 10 (mean difference -17.28 ml, 95% CI: -52.01, 17.44, P=0.33). CONCLUSION On the basis of the current evidence, EAT seems to be an effective marker in the prediction of CAD.

Polysaccharide from seeds of Plantago asiatica L. increases short-chain fatty acid production and fecal moisture along with lowering pH in mouse colon.

Abstract: Mice (20.0 ± 2.0 g, n = 48 per group) were given 30 days oral administration of polysaccharide from Plantago asiatica L. seeds at the dose of 0.4 g/kg body weight by gavage to investigate the effects of the polysaccharide on mouse colon. Results showed that the concentrations of total short-chain fatty acids (SCFA), acetic, propionic, and n-butyric acids in mouse colonic content of polysaccharide treated group were all significantly higher than that of control group (water) (p < 0.05). In addition, moisture of mouse colonic content of polysaccharide treated group was also notably higher than that of the control group (p < 0.05) indicating the intake of polysaccharide from P. asiatica L. resulted in a stronger water-holding capacity for colonic content throughout the experimental period. Furthermore, a decreased pH (from 7.5 ± 0.1 to 7.2 ± 0.1) was observed in mouse colon of the polysaccharide treated group compared with the control group (pH from 7.5 ± 0.1 to 7.5 ± 0.1). These results suggested that the intake of the polysaccharide from P. asiatica L. might be beneficial for the colon health.

Controllable deposition of a platinum nanoparticle ensemble on a polyaniline/graphene hybrid as a novel electrode material for electrochemical sensing.

Abstract: We demonstrate for the first time an interfacial polymerization method for the synthesis of high-quality polyaniline-modified graphene nanosheets (PANI/GNs), which represents a novel type of graphene/polymer heterostructure. The interfacial polymerization at a liquid-liquid interface allows PANI to grow uniformly on the surface of the GNs. An ultra-high loading of Pt nanoparticles was then controllably deposited on the surface of the PANI/GNs to form a Pt/PANI/GNs hybrid. The obtained composites were characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy, and thermogravimetric analysis. The Pt/PANI/GNs hybrid shows excellent electrocatalytic activity toward methanol oxidation and oxygen reduction. H(2)O(2) and glucose were used as two representative analytes to demonstrate the sensing performance of a Pt/PANI/GNs-modified electrode. It is found that this sensing element shows high sensitivity and a low detection limit for H(2)O(2) and glucose. The results demonstrate that the Pt/PANI/GNs hybrid may be an attractive and advanced electrode material with potential applications in the construction of electrochemical sensors and biosensors.

Spectroscopic studies of DNA interactions with food colorant indigo carmine with the use of ethidium bromide as a fluorescence probe.

Abstract: The interaction of indigo carmine (IC) with calf thymus DNA in physiological buffer (pH 7.4), using ethidium bromide (EB) dye as a fluorescence probe, was investigated by ultraviolet–visible absorption, fluorescence, and circular dichroism (CD) spectroscopy, coupled with viscosity measurements and DNA-melting studies. Hypochromicity of the absorption spectra of IC and enhancement in fluorescence polarization of IC were observed with the addition of DNA. Moreover, the binding of IC to DNA was able to decrease iodide and single-stranded DNA (ssDNA) quenching effects, increase the melting temperature and relative viscosity of DNA, and induce the changes in CD spectra of DNA. All of the evidence indicated that IC interacted with DNA in the mode of intercalative binding. Furthermore, the three-way synchronous fluorescence spectra data obtained from the interaction between IC and DNA–EB were resolved by parallel factor analysis (PARAFAC), and the results provided simultaneously the concentration information and...

Electron acceleration in the reconnection diffusion region: Cluster observations

Abstract: We present one case study of magnetic islands and energetic electrons in the reconnection diffusion region observed by the Cluster spacecraft. The cores of the islands are characterized by strong c ...