Novozymes

About: Novozymes is a company organization based out in Copenhagen, Denmark. It is known for research contribution in the topics: Nucleic acid & Polynucleotide. The organization has 2506 authors who have published 2828 publications receiving 89266 citations. The organization is also known as: Novo Enzymes A/S & Novozymes A/S.

Production of savinase and population viability of Bacillus clausii during high-cell-density fed-batch cultivations.

Abstract: The growth and product formation of a Savinase-producing Bacillus clausii were investigated in high-cell-density fed-batch cultivations with both linear and exponential feed profiles. The highest specific productivity of Savinase was observed shortly after the end of the initial batch phase for all feed profiles applied and, in addition, there was a time-dependent decrease in specific productivity. The specific glucose uptake rate increased with time for constant specific growth rate indicating that the maintenance requirements increased with time, possibly due to a decreasing K+ concentration. The physiological state of the cells was monitored during the cultivations using a flow cytometry assay based on the permeability of the cell membrane to propidium iodide. In the latter parts of the fed-batch cultures with a linear feed profile, a large portion of the cell population was found to have a permeable membrane, indicating a large percentage of dead cells. By assuming that only cells with a nonpermeable membrane contributed to growth and product formation, the physiological properties of this subpopulation were calculated. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 344–352, 2003.

Enzymatic processes and enzyme development in biorefining

Abstract: Improvements in enzyme cocktails for converting biomass have driven down costs for production of bioproducts and have allowed commercialization of the first cellulosic biorefineries. Innovations in biochemical engineering have resulted in cocktails that require dramatically less enzyme for a given level of biomass conversion than were required just a few years ago. Developments in the enzymes themselves must be accompanied by optimization of the biochemical conversion process to allow for the lowest possible product costs to be achieved. This chapter describes both enzyme innovations and critical themes in process optimization.

Lipolytic enzyme variants and method for their production

Abstract: The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.

Methods, compositions and systems for controlling fouling of a membrane

Abstract: The present invention provides methods and compositions for improving permeability and flux in a membrane filtration system, especially in water or wastewater treatment processes.

Stable yeast 2 mu m vector

Abstract: A 2 mu m plasmid vector for transforming yeast, especially brewing yeast, much which comprises a DNA sequence allowing propagation of the plasmid in bacteria, two copies of the 74 base pair FLP recombination site of the 2 mu m plasmid in direct orientation and a DNA sequence coding for a protein or peptide of interest The plasmid is so constructed that in yeast the bacterial DNA sequence is between the two recombination sites which are in direct orientation and is spontaneously lost following recombination A "gene of interest" is preferable inserted at the SnaBI site