Randall Division of Cell and Molecular Biophysics

About: Randall Division of Cell and Molecular Biophysics is a based out in . It is known for research contribution in the topics: Actin cytoskeleton & Skeletal muscle. The organization has 576 authors who have published 1229 publications receiving 78279 citations.

An overview of molecular aspects of iron metabolism

Abstract: Iron (Fe) is an essential, but potentially noxious, metal for almost all organisms Its precise cellular regulation is necessary to ensure synthesis of numerous iron-containing proteins required for metabolic processes yet at the same time avoiding the build-up of potentially toxic levels of iron In humans, iron-deficiency results in anemia, while excess iron can lead to organ damage as a result of a build-up of non-transferrin-bound iron (NTBI) In recent years, the cloning of novel proteins has clarified the mechanisms of iron uptake, storage and metabolic regulation Our current knowledge of the molecular aspects of mammalian iron metabolism and NTBI are presented in this review

High Resolution Analysis of the Chromatin Landscape of the IgE Switch Region in Human B Cells

Abstract: Antibodies are assembled by a highly orchestrated series of recombination events during B cell development. One of these events, class switch recombination, is required to produce the IgG, IgE and IgA antibody isotypes characteristic of a secondary immune response. The action of the enzyme activation induced cytidine deaminase is now known to be essential for the initiation of this recombination event. Previous studies have demonstrated that the immunoglobulin switch regions acquire distinct histone modifications prior to recombination. We now present a high resolution analysis of these histone modifications across the IgE switch region prior to the initiation of class switch recombination in primary human B cells and the human CL-01 B cell line. These data show that upon stimulation with IL-4 and an anti-CD40 antibody that mimics T cell help, the nucleosomes of the switch regions are highly modified on histone H3, accumulating acetylation marks and tri-methylation of lysine 4. Distinct peaks of modified histones are found across the switch region, most notably at the 5' splice donor site of the germline (I) exon, which also accumulates AID. These data suggest that acetylation and K4 tri-methylation of histone H3 may represent marks of recombinationally active chromatin and further implicates splicing in the regulation of AID action.

Components of therapy as mechanisms of change in cognitive therapy for people at risk of psychosis: analysis of the EDIE-2 trial.

Abstract: This is an author-produced electronic version of an article accepted for publication in the British Journal of Psychiatry. The definitive publisher-authenticated version is available online at http://bjp.rcpsych.org

Crystal structure of ribosomal protein L30e from the extreme thermophile Thermococcus celer: thermal stability and RNA binding.

Abstract: We report here the high-resolution crystal structure of the ribosomal protein L30e from the hyperthermophilic archaeon Thermococcus celer determined at cryo-temperature When it is compared with its mesophilic homologue, L30e from yeast, a number of structural features that can enhance thermostability are revealed Disordered residues corresponding to a large RNA-binding loop in yeast L30e are well structured in the T celer protein The overall charge of T celer L30e is near neutral, whereas that of the yeast homologue is highly positive This is the result of an increase in the number of acidic residues at the expense of polar residues, Asn, Ser, and Thr Extensive ion pair networks are found on the molecular surface Exposed nonpolar surface areas are reduced in the T celer protein Its side chain atoms preferably form hydrogen bonds with main chain atoms Taken together, these factors contribute to high protein stability The roles of well-conserved L30e residues are studied and found to be important in defining a very compact overall structure and in maintaining the structure of the RNA binding site By comparing it with the yeast homologue, we also identified the residues that are responsible for RNA binding and built a model to illustrate how L30e binds to an RNA kink turn motif