Institution
Rural Development Administration
Government•Jeonju, South Korea•
About: Rural Development Administration is a government organization based out in Jeonju, South Korea. It is known for research contribution in the topics: Gene & Population. The organization has 4372 authors who have published 4919 publications receiving 94318 citations.
Topics: Gene, Population, Genome, Quantitative trait locus, Arabidopsis
Papers published on a yearly basis
Papers
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TL;DR: A 38-mer mature peptide was chemically synthesized via the Fmoc method, and its antimicrobial activity was analyzed, and it evidenced a broad spectrum of activity against fungi, Gram-positive and Gram-negative bacteria, and also evidenced no hemolytic activity against human red blood cells.
Abstract: Cecropin is a well-studied antimicrobial peptide that is synthesized in fat body cells and hemocytes of insects in response to hypodermic injury or bacterial infection. A 503 bp cDNA encoding for a cecropin-like peptide was isolated by employing annealing control primer (ACP)-based differential display PCR and 5′-RACE with immunized Papilio xuthus larvae. The open reading frame of the isolated cDNA encoded for a 62-amino acid prepropeptide with a putative 22-residue signal peptide, a 2-residue propeptide, and a 38-residue mature peptide with a theoretical mass of 4060.89 Da. The deduced amino acid sequence of the peptide evidenced a significant degree of identity with other lepidopteran cecropins. This peptide was named papiliocin. RTPCR results revealed that the papiliocin transcript was detected at significant levels after injection with bacterial lipopolysaccharide (LPS). On the basis of the deduced amino acid sequence of papiliocin, a 38-mer mature peptide was chemically synthesized via the Fmoc method, and its antimicrobial activity was analyzed. The synthetic papiliocin peptide evidenced a broad spectrum of activity against fungi, Gram-positive and Gram-negative bacteria, and also evidenced no hemolytic activity against human red blood cells.
52 citations
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TL;DR: expression of castor bean LEC2 in Arabidopsis increased the expression of fatty acid elongase 1 (FAE1) and induced the accumulation of triacylglycerols, especially those containing the seed‐specific fatty acid, eicosenoic acid, in vegetative tissues.
52 citations
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TL;DR: This study is the first to show Agrobacterium-mediated transformation in bottle gourd, and genetic segregation analysis of T1 progenies showed that transgenes were inherited in a Mendelian fashion.
Abstract: We describe a procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 that carries the binary vector pCAMBIA3301 containing a glufosinate ammonium-resistance (bar) gene and the β-d-glucuronidase (GUS) reporter gene. The most effective bacterial infection was observed when cotyledon explants of 4-day-old seedlings were co-cultivated with Agrobacterium for 6–8 days on co-cultivation medium supplemented with 0.1–0.001 mg/l l-α-(2-aminoethoxyvinyl) glycine (AVG). The putatively transformed shoots directly emerged at the proximal end of cotyledon explants after 2–3 weeks of culturing on selection medium containing 2 mg/l dl-phosphinothricin. These shoots were rooted after 3 weeks of culturing on half-strength MS medium containing 0.1 mg/l indole acetic acid and 1 mg/l dl-phosphinothricin. Transgenic plants were obtained at frequencies of 1.9%. Stable integration and transmission of the transgenes in T1 generation plants were confirmed by a histochemical GUS assay, polymerase chain reaction and Southern blot analyses. Genetic segregation analysis of T1 progenies showed that transgenes were inherited in a Mendelian fashion. To our knowledge, this study is the first to show Agrobacterium-mediated transformation in bottle gourd.
52 citations
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TL;DR: The ITS rDNA sequence and morphological data demonstrated that P. farinosa from S. oleracea is distinct from downy mildew of other chenopodiaceous hosts, and the name Peronospora effusa should be reinstated for the downy Mildew fungus found on spinach.
52 citations
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TL;DR: Survivals and growth of pathogens on vegetables were different depending on the pathogen type and storage temperature, therefore, vegetables should be stored under refrigerated conditions (below 4 °C) prior to consumption.
Abstract: We conducted this study to investigate the survival and growth of pathogens on fresh vegetables stored at 4 and 15 °C. Vegetables (romaine lettuce, iceberg lettuce, perilla leaves, and sprouts) were inoculated with 4 pathogens (Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7) and stored at 2 different temperatures for different periods of time (3, 6, 9, 12, and 15 d at 4 °C and 1, 2, 3, 5, and 7 d at 15 °C). Populations of the 4 pathogens tended to increase on all vegetables stored at 15 °C for 7 d. Populations of E. coli O157:H7 and S. Typhimurium increased significantly, by approximately 2 log10CFU/g, on loose and head lettuce stored at 15 °C for 1 d. No significant differences were observed in the growth of different pathogens on vegetables stored at 4 °C for 15 d. E. coli O157:H7 did not survive on sprouts stored at 15 or 4 °C. The survival and growth of food pathogens on fresh vegetables were very different depending on the pathogen type and storage temperature.
Practical Application: Survivals and growth of pathogens on various vegetables at 4 and 15 °C were observed in this study. Survivals and growth of pathogens on vegetables were different depending on the pathogen type and storage temperature. Therefore, vegetables should be stored under refrigerated conditions (below 4 °C) prior to consumption. This recommendation may vary depending on the type of vegetable.
52 citations
Authors
Showing all 4390 results
Name | H-index | Papers | Citations |
---|---|---|---|
Richard G. F. Visser | 85 | 607 | 31019 |
Sung Woo Kim | 60 | 319 | 12280 |
Ill-Min Chung | 57 | 539 | 12573 |
Kwang-Jin Kim | 50 | 244 | 7629 |
Jules Janick | 45 | 348 | 9359 |
Pil Joon Seo | 45 | 121 | 6799 |
Sun Yeou Kim | 44 | 148 | 5441 |
Tae-Jin Yang | 42 | 209 | 9847 |
Mariadhas Valan Arasu | 41 | 252 | 5545 |
Hyeran Kim | 41 | 198 | 14548 |
Muhammad Rauf | 41 | 164 | 6742 |
Yong Pyo Lim | 41 | 226 | 8325 |
Sang Hong Lee | 39 | 97 | 11171 |
Young Jun Kim | 36 | 233 | 5498 |
Gi-Ho Sung | 36 | 168 | 12702 |