Showing papers in "FEBS Open Bio in 2014"

TL;DR: These data provide mechanistic insights into primary chemoresistance in HCC by showing that TGFβ selectively enriches linc‐RoR within extracellular vesicles, which has a potential role in intercellular signaling in response to TGF β, and support targeting l inc‐ROR to enhance chemosensitivity in H CC.

TL;DR: It is demonstrated that miR34a over‐expression in glioma stem cells profoundly decreased levels of p‐AKT (Ser473), increased GSK‐3β levels and targeted for degradation β‐catenin, an important mediator of Wnt signaling pathway.

TL;DR: It is shown that RIPK1 phosphorylation, which is a hallmark of RIPK‐dependent necrosis, was induced by APAP, and the expression pattern in the liver overlapped with that of CYP2E1, whose activity around the central vein area has been demonstrated to be critical for the development of APAP‐induced hepatic injury.

TL;DR: The results provide a direct evidence for a carotenoid origin of these compounds and point to CCD1s as the enzymes catalyzing the formation of the vast majority of tomato isoprenoid volatiles, many of which are aroma constituents.

TL;DR: Findings suggest that targeted inhibition of HDAC may be used as a viable therapeutic strategy for disrupting tumor resistance caused by NFκB, and epigenetic modifications of HNSCC resulting fromNFκB‐induced histone modifications constitute a novel molecular mechanism responsible for chemoresistance in H NSCC.

TL;DR: The new FAP enzyme assay is the first to be thoroughly characterised and shows that FAP activity is measurable in most organs and at high levels in some, particularly liver fibrosis.

TL;DR: An in situ proximity ligation assay was used to confirm whether AhR was translocated to the nucleus alone or together with HSP90, as shown by the transcription induction of the gene CYP1A1 8 h after treatment with β‐NF.

TL;DR: It is observed that ebselen exposure causes an increase in intracellular ROS levels and mitochondrial membrane potential, and that these effects were reversed by addition of antioxidants such as reduced glutathione (GSH) or N‐acetyl‐l‐cysteine (NAC).

TL;DR: It is confirmed that autophagy has cytoprotective role and is activated in mutually exclusive manner with respect to ER stress levels and the mathematical model shows that both the activation threshold and temporal dynamics of Autophagy and apoptosis inducers are sensitive to variation in mTOR activity.

TL;DR: This is the first report showing the dual binding (chromosomal DNA and core histones) property of a classical intercalator, EB, and its longer analogue, PI, in the context of chromatin.

TL;DR: It is shown that stabilizing disulfide bonds do not have to span many residues, as the most stabilizingdisulfide bond, L323C–A325C, spans only one residue while it stabilizes the enzyme, as shown by a 6 °C increase in its apparent melting temperature.

TL;DR: The shared downstream RNA targets of TDP‐43 and FUS may form a common pathway in the neurodegenerative processes of ALS/FTLD, and this work assessed the differential gene expression and alternative splicing profiles related to regulation by either TDP or FUS in primary cortical neurons.

TL;DR: Plant flavonoid quercetin‐3‐β‐d‐glucoside (Q3G) may represent an effective anti‐cholesterolemic agent as an LDLR inducer and a PCSK9 anti‐secretagogue.

TL;DR: A novel signaling pathway by which curcumin induces ROS‐dependent Par‐4 activation and ceramide generation, leading to autophagic cell death in human malignant glioma cells is described.

TL;DR: expression of castor bean LEC2 in Arabidopsis increased the expression of fatty acid elongase 1 (FAE1) and induced the accumulation of triacylglycerols, especially those containing the seed‐specific fatty acid, eicosenoic acid, in vegetative tissues.

TL;DR: A novel role is reported of this highly immunogenic PE25/PPE41 protein complex in inducing necrosis, but not apoptosis, in macrophages and it is proposed that these protein complexes of M. tuberculosis, secreted by similar/unique transport system (Type VII), have an important role in M.culosis virulence and disease reactivation.

TL;DR: An ancestral sequence of proto‐tRNAs is built that shows a remarkable overall identity with the catalytic site of PTC, concluding that the Peptidyl Transferase Center was indeed originated by the fusion of ancestral sequences of proto-tRNA.

TL;DR: The nucleolar presence of miRNAs was independent of Dicer and the main activity of the nucleolus, RNA polymerase I transcription, but was dependent on CRM1 previously associated with nucleolar trafficking of small nucleolar RNAs.

TL;DR: This study indicates that conditional targeting can be accomplished effectively using paired Cas9n and a single DNA template.

TL;DR: It is document that SND1 increases angiotensin II type 1 receptor (AT1R) levels by increasing AT1R mRNA stability, which results in activation of ERK, Smad2 and subsequently the TGFβ signaling pathway, promoting epithelial–mesenchymal transition (EMT) and migration and invasion by human HCC cells.

TL;DR: Overexpression of aromatic catabolic genes in strain PNB, revealed by real‐time PCR analyses, is a way forward to understand the complex regulation of degradative genes in sphingomonads.

TL;DR: The reconstructed brain model enables the elucidation of effects of a perturbation on brain metabolism and the illumination of possible machineries in which a specific metabolite or pathway acts as a regulatory spot for cellular reorganization.

TL;DR: This comprehensive characterization of mRNA and miRNA transcriptomes in NPC cell lines and the xenograft provides insights on miRNA regulation of RNA and valuable resources on transcript variation and regulation in NPC, which are potentially useful for mechanistic and preclinical studies.

TL;DR: Results indicate that autophagy in RPE cells is a vital cytoprotective process that prevents the accumulation of damaged cellular molecules.

TL;DR: The results suggest that high levels of oxidative stress due to concomitant deficiency of SMP30 and/or AA, and SOD1 cause abnormal plasma lipid metabolism, hepatic lipid accumulation and premature death resulting from impaired VLDL secretion.

TL;DR: Using mass spectrometry, it is demonstrated that HYAL1 was N‐glycosylated at the three asparagine residues, and its secretion and enzymatic activity are regulated by N‐ Glycosylation.

TL;DR: The results suggest that the diminished expression of h2‐calponin in prostate cancer cells increases cell motility, decreases substrate adhesion, and promotes adhesion on high stiffness substrates.

TL;DR: The yeast Saccharomyces cerevisiae IMA multigene family encodes four isomaltases sharing high sequence identity from 65% to 99%.

TL;DR: It is demonstrated that disruption of the specific HSP20–PDE4D interaction leads to attenuation of pathological cardiac remodelling.

TL;DR: Data suggest that salmon STAT2 and IRF9 may have a role in IFNγ‐induced signaling and promote the expression of GAS‐driven genes in bony fish.