Journal ArticleDOI
Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure.
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From the correlation of the gel bands with 12 isolated polypeptides from which the complete amino acid sequence is known, it is concluded that mammalian cytochrome c oxidase consists of 13 different polypeptin which can all be separated by the described procedure.About:
This article is published in Analytical Biochemistry.The article was published on 1983-03-01. It has received 432 citations till now. The article focuses on the topics: Cytochrome c oxidase & Sodium dodecyl sulfate.read more
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Journal ArticleDOI
Deficiencies in Complex I subunits of the respiratory chain in Parkinson's disease
Yoshikuni Mizuno,Shigeo Ohta,Masashi Tanaka,Shinzaburo Takamiya,Keiji Suzuki,Takeshi Sato,Hiroshi Oya,Takayuki Ozawa,Yasuo Kagawa +8 more
TL;DR: No clear difference was noted in immunoblotting studies on subunits of Complexes III and IV between the control and Parkinson's disease, but defects in Complex I subunits seem to be one of the most important clues to elucidate pathogenesis of Parkinson's Disease.
Journal ArticleDOI
Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes☆
TL;DR: Human blood mononuclear cells were cultured for 24 h in the presence of LPS, and a monocyte-derived neutrophil-activating factor (NAF) was purified to apparent homogeneity from the conditioned media.
Book ChapterDOI
Oxidative phosphorylation diseases. Disorders of two genomes.
TL;DR: The mitochondrial cytopathies are a heterogeneous group of diseases associated with defects in mitochondrial ATP production that affect the brain, skeletal muscle, heart, kidney, and liver.
Journal ArticleDOI
Cytochrome c oxidase deficient fibres in the limb muscle and diaphragm of man without muscular disease: An age-related alteration
TL;DR: The results indicate that cytochrome c oxidase deficient muscle fibres in normal skeletal muscle represent an age related phenomenon which probably results from cellular ageing and might be involved in the reduction of muscle mass and strength during senescence.
Journal Article
Cytochrome-c-oxidase deficient cardiomyocytes in the human heart--an age-related phenomenon. A histochemical ultracytochemical study.
TL;DR: The results indicate that cytochrome-c-oxidase deficient heart muscle cells represent a degenerative lesion associated with cellular ageing and may be involved in the reduction of myocardial contractile ability in senescence.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels
TL;DR: The number of steps in the procedure is reduced from 10 to 6, the reagents in each step are simplified, and the amount of silver required is reduced by a factor of 10, thus greatly reducing the expense of the procedure.
Journal ArticleDOI
Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.
Journal ArticleDOI
A two-subunit cytochrome c oxidase (cytochrome aa3) from Paracoccus dentrificans
Bernd Ludwig,Gottfried Schatz +1 more
TL;DR: Paracoccus cytochrome c oxidase (PCO) as discussed by the authors contains two heme groups (a and a3) and two copper atoms per minimal unit, and when incorporated into liposomes, generates an electrochemical proton gradient during the enzyme activation.