Showing papers in "Biochimie in 1996"

TL;DR: A World Wide Web version of the sequence retrieval system Query: WWW-Query is developed, which allows to query nucleotide sequence banks in the EMBL/GenBank/DDBJ formats and protein sequence Banks in the NBRF/PIR format.

TL;DR: It is found that IbpA/Bs also recognize endogenous bacterial proteins aggregated intracellularly by heat shock, and in the E coli rpoH strain the level of IbPA/B was higher than in wild type before the heat shock and rose to still higher levels after it, pointing to a regulation of ibpA /B operon by another factor, besides that of sigma 32.

TL;DR: There is now considerable evidence that a specific site (or sites) in the 5' leader region of all retroviruses, located either upstream or/and downstream of the major splice donor site, is involved in the dimer linkage.

TL;DR: A system called MAGPIE (Multipurpose Automated Genome Project Investigation Environment) has been designed and implemented to meet the challenges of automated whole genome analysis and performs reliably on local UNIX workstations and communicates with remote resources through standard networking protocols.

TL;DR: A model is presented for the regulation of the double-stranded RNA-activated mammalian protein kinase PKR, which is involved in protein synthesis inhibition and the antiviral response in cells and includes the suggestion that PKR dimers form which are stabilized and rearranged upon binding to dsRNA regions 60 bp or longer.

TL;DR: Inosine-34 and N1-methylinosine-37 in human tRNA(Ala) are targets for specific autoantibodies which are present in the serum of patients with inflammatory muscle disease of the PL-12 polymyositis type.

TL;DR: The results indicate that faithful processing at both ends of the snoRNAs can be uncoupled and that it is not strictly dependent on pre-mRNA splicing.

TL;DR: A simple adaptation of Ninio's vectorial representation of DNA sequences for the detection of replication origins in bacteria is presented, outlining the origins of replication in Escherichia coli, Bacillus subtilis, Haemophilus influenzae and Mycoplasma genitalium.

TL;DR: The first direct observations of interaction between Pdx and CYP101 are described, and results of the NMR experiments indicate that conformational gating of the electron transfer complex between CYP 101 and Pdx may be important.

TL;DR: It is shown that nucleolin extracted from human, hamster and mouse cells interacts with the same specificity and affinity to a mouse 5'ETS RNA fragment which contains a NRE motif, which suggests that these nucleolin binding sites might be functionally important for ribosome biogenesis.

TL;DR: Comparisons with other sequences in the data base suggest that some of the eIF3 subunits may have functions apart from the eif3 complex, and work is in progress to use the cloned DNAs as tools for elucidating the structure of eIF2 and its interactions with other initiation factors.

TL;DR: No significant mitochondrial co-import of the normally cytosol-specific tRNA(Asp) was detected in transgenic plants expressing the yeast cytosolic aspartyl-t RNA synthetase fused to a mitochondrial targeting sequence, suggesting that, although necessary, recognition by a cognate aminoacyl-tRNA synthetases might not be sufficient to allow tRNA import into plant mitochondria.

TL;DR: Glutamine metabolism in C6 cells appears complementary to that of glucose as far as energy production and carbon sources for the growing of the cells are concerned: glutamine is mainly utilized for anaplerosis as carbon donor to replenish the tricarboxylic acid cycle; it is not a substrate for energy metabolism.

TL;DR: It is concluded that E coli RF-2 activity is potentially weak and that the amino acid at position 246 plays a crucial role, not for codon discrimination, but for stop codon recognition or polypeptide release, presumably constituting an essential moiety of tRNA mimicry or interacting with peptidyltransferase centers of the ribosome.

TL;DR: The data supporting the two functions of ribosome recycling factor (RRF) suggest that RRF participates not only in the disassembly of the termination complex but also in peptide elongation.

TL;DR: It is shown that their large size correlates with their ability to perform complex tasks and how the ribozyme activity of group II introns might be involved in their transposition at the DNA level is recalled.

TL;DR: A series of variant crp terminators were constructed and their abilities regarding transcription termination and mRNA stabilization were analyzed, showing that the contribution of the G(+)C-rich dyad symmetry sequence to transcription termination is larger than that of the poly(T) tract.

TL;DR: The translational stop signal is deduced to be an extended factor recognition sequence with a core element, rather than simply a factor recognition triplet codon influenced by context.

TL;DR: In vivo and in vitro import assays were developed to study the mechanisms of tRNA-K1 mitochondrial import and possible mechanisms of intracellular partitioning and mitochondrial membrane translocation of tRNAs are discussed.

TL;DR: This review focuses on recent data concerning the properties of the initiator tRNA and of enzymes and factors involved in the translation initiation process.

TL;DR: The refined crystal structure of the ternary complex of yeast Phe-tRNAPhe, Thermus aquaticus elongation factor EF- Tu and the non-hydrolyzable GTP analog, GDPNP, reveals many details of the EF-Tu recognition of aminoacylated tRNA (aa-tRNA).

TL;DR: The cDNAs and genes for two isozymes of cytochrome P450nor of the fungus Cylindrocarpon tonkinense were cloned and sequenced, and the specificity of chimeric proteins against NADH/NADPH showed that the specificity is determined by the N-terminal half of protein.

TL;DR: The presence of folded regions in RNA competes with the binding of a complementary oligonucleotide, resulting in a weak antisense effect, which might be of interest to design antisense sequences able to selectively interact with such motifs in order to interfere with the biological processes they mediate.

TL;DR: Preparations of nitric oxide synthases from porcine cerebellum, bovine aortic endothelium and cytokine-treated murine macrophages were inhibited by PN, suggesting the irreversible oxidation of this bond may be the common mechanism of NOS inhibition.

TL;DR: A novel approach to genetic sequence analysis based on compression of algorithms, which can be applied to a sequence in order to study the presence of those regularities all over the sequence.

TL;DR: Bacteriophage lambda virions whose tail tube major subunit (V) proteins are modified with a cyclizable Arg-Gly-Asp (RGD) peptide are able to promote the binding of certain mammalian cells to a solid surface, and phage-mediated transfection benefits when the efficiency of only one step in the multi-stage uptake process is improved.

TL;DR: The liquid crystal properties of Congo red enable it to form specific complexes with proteins, which have become accessible to penetration by ligands after global or local disruption of tertiary interaction, and this dye may thus be used as a stabilizer of unfolding intermediates in the step preceding the molten globule state.