Showing papers in "Cryobiology in 1992"

TL;DR: A difference in both composition and thermotropic phase behavior of glycolipids between rooster and mammalian sperm is demonstrated which may be related to the greater tolerance of rooster sperm to rapid cooling.

TL;DR: In this article, the authors used subambient thermal analysis with differential scanning calorimetry and thermomechanical analysis to optimize the lyophilization of proteins based on the thermal behavior of solution components.

TL;DR: The potential value of using available genetic models (lines of roosters differing in the capacity of their sperm to survive a freeze-thaw cycle) to clarify and overcome damage to poultry sperm induced by cryopreservation are discussed.

TL;DR: Transfer of four embryos (two morulae and two blastocysts) derived from post-IVM S-oocytes into a recipient heifer resulted in pregnancy and the birth of twin calves.

TL;DR: It is reasonable that, among the disaccharides studied, trehalose exhibits the maximum stabilizing effect on the bilayer structure of lipid whose acyl chains are bonded with each other by the ap polar interaction, because the apolar interaction is strengthened with the stabilization of the surrounding water structure.

TL;DR: It is reported here that the addition of fish antifreeze glycopeptides (AFGPs) to vitrifying solutions increases post-thaw viability in cultured immature pig oocytes and two-cell stage embryos of mice and pigs after rapid cooling to cryogenic temperatures.

TL;DR: Doubling generation lengths for callitrichid primates through use of cryopreservation could almost triple the number of species that could be rescued from extinction in world zoos.

TL;DR: The data support the hypothesis that the freeze tolerance exhibited by A. coronifer is closely related to its ability to be in the state of anhydrobiosis, as it is similar to the survival of animals cooled to −196 °C at a constant rate of 30 °C min −1.

TL;DR: Functional assessments showed that human hepatocytes which survive freezing and thawing preserve their protein synthesis capabilities and are able to secrete a specific protein, anionic peptidic fraction, which is involved in the hepatic uptake of bile-destined cholesterol.

TL;DR: DMSP may be a compatible osmotic solute only under conditions of moderate temperature and low, yet physiological, concentrations and may be an effective protein cryoprotectant in the evolutionary selection of organic osmolytes.

TL;DR: The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy and a computer model was used to derive values for membrane water permeability (Lp) and inactive volume (Vb) and to compare the experimental data to the predicted values.

TL;DR: Embryos were rendered anoxic prior to chilling, a treatment that has been shown by Foe and Alberts to reversibly halt development of early stages and the computed curve of survival vs temperature agrees closely with observed survivals.

TL;DR: The discovery of glycerol as an effective cryoprotectant for spermatozoa led to research on cryopreservation of embryos, and recent research has shown that embryos may also be frozen by a "nonequilibrium" method.

TL;DR: For all species tested, there was a window of desiccation during which survival through freezing was maximized, and differences in the level of survivalThrough freezing during this window ranged from complete survival of A. hippocastanum to survival and growth of the root with C. sativa, and callus growth from Quercus sp.

TL;DR: The effective BHT analogs, if partitioned into the membrane, are indistinguishable with regard to their capacity to eliminate cold-induced membrane lysis; (b) membrane-linked events are uniquely disrupted by a subset of this analog family; and (c) when concentrations of egg yolk and B HT analogs are carefully controlled, unique synergistic effects are noted.

TL;DR: The results show that PROH can induce degeneration and parthenogenetic activation in the mouse oocyte in a concentration, temperature, and time-dependent way and that the use of PROH as a single cryoprotectant for the freezing of mature unfertilized oocytes cannot be recommended in procedures involving ambient temperature or concentrations exceeding 1.5 M PROH.

TL;DR: Cryopreservation of axillary shoot-tips of pear in vitro cultures was performed after encapsulation in alginate beads and the best results (80% shoot recovery) were obtained using 0.75 M sucrose for preculture and 4-h dehydration (giving 20% residual water).

TL;DR: The use of intermittent pulses of high temperature could be exploited during cold storage of insects to maintain protection against cold shock injury and naturally occurring temperature cycles may sustain cold tolerance in a similar manner.

TL;DR: Frogs subjected to experimental hibernation and immunized showed weakened immune responses when brought from the hibernaculum, suggesting the apparent lymphocyte aplasia may contribute to the absence of immunological responsiveness during periods of hibernation.

TL;DR: The permeability coefficient of glycerol (Pg) and its activation energy (Ea) were determined by measuring the time required for 50% spermolysis in hyperosmotic glycersol solutions which were hypotonic with respect to electrolytes.

TL;DR: The results show that the critical cooling rates of butane-2,3-diol and glycerol are reduced by varying amounts by the addition of such materials but that the reduction is not as great as is achieved by the additions of polyethylene glycol with a molecular weight of 400.

TL;DR: It is demonstrated that liquid nitrogen storage of valve bioprostheses is required for long-term preservation of cellular functions.

TL;DR: It seems that chemotherapy may be more effective after cryotherapy, and a multicenter study is in progress to evaluate the association of cryo-chemotherapy in France.

TL;DR: It was observed, however, that the presence of secondary branches in the ice phase correlated with lower survival for a given cooling rate, indicating that not only is the redistribution of solute during freezing a potential source of damage during freezing but ice/cell interactions are also.

TL;DR: Although the cryostorage method described in the present report provided only limited preservation of human coronary arteries, significant vascular smooth muscle and endothelial-dependent functions were retained and it is possible that further refinement of the present cryost Anchorage methodology may provide better preservation of functionally viable human blood vessels.

TL;DR: The results indicate that sperm survival in vitro at 3 degrees C was significantly enhanced by 20 mM K+ in Tyrode's solution relative to TyroDe's with less K+.

TL;DR: This study represents a first attempt to predict intracellular water loss from ram sperm during cooling and may provide a novel approach for the interpretation of the many empirical studies carried out to investigate optimal conditions for the cryopreservation of sperm.

TL;DR: Permeabilized embryos are permeable to water, ethylene glycol, glycerol, and the stain rhodamine B (which was used to assess permeabilization) and they are effectively impermeable to sucrose.

TL;DR: Embryonic axes of Quercus faginea were tested for viability after rapid freezing and storage in liquid nitrogen (−196 °C) and best germination response after freezing was observed when embryonic axes were desiccated from 53 to 21% moisture content (on a fresh weight basis).

TL;DR: A simple and safe procedure is suggested for the addition of Me2SO to hepatocytes in a sandwich culture configuration and its subsequent removal, which will be valuable for studies on hepatocyte cryopreservation.