Showing papers in "Experimental Parasitology in 1983"

TL;DR: To evaluate rapidly Plasmodium falciparum growth in Vitro, [3H]hypoxanthine was added to parasite microcultures and radioisotope incorporation was measured and it was found that incorporation was proportional to the number of parasitized erythrocytes present.

TL;DR: Resistance of mice to reinfection with Schistosoma mansoni has been demonstrated by a reduction in the recovery of challenge worms from the lungs, liver, and mesenteric veins, however, histological examination of the skin and lungs have failed to identify the major site(s) of challenge worm elimination.

TL;DR: The effectiveness of various dosages of different species/strains of nematodes was compared for Galleria mellonella and various pest insects that live in or pupate in soil and no onespecies/strain was the most infective for all insect species.

TL;DR: It is suggested that proteolytic activity may be important both in the rupture of schizont-infected erythrocytes and in the invasion of ery Throcytes by malaria parasites.

TL;DR: Each of five Neoaplectana (Nematoda: Steinernematidae) species was cultured monoxenically with various Xenorhabdus isolates and a high proportion of infectives were able to carry within their intestine X. nematophilus isolated from other strains of the same nematode species.

TL;DR: It is proposed that some parasites in continuous culture may not express the molecules responsible for binding, although the morphologic knobs are still present.

TL;DR: A metalloprotein with superoxide dismutase activity was isolated and purified from muscle-stage Trichinella spiralis and may function as an essential defense mechanism against the highly destructive superoxide radical encountered either intracellularly, as a product of biological oxidation, or externally, asA component of the host's immune system.

TL;DR: The number of trophozoites in the small intestine and cyst excretion were found to be proportional at all stages of infection, and there was a direct correlation between the size of inoculum and the length of the latent period.

TL;DR: Intact trophozoites of the virulent Entamoeba histolytica strain HM-1:IMSS (HM-1) destroyed a monolayer of baby hamster kidney (BHK) cells at a higher rate and efficiency than trophozite of the nonvirulent strain HK-9.

TL;DR: It was concluded that for a given serodeme the variable antigen composition of the metacyclic population which develops in the tsetse is constant and characteristic and it may be possible to use the chancre as a marker forserodeme analysis.

TL;DR: There is the possibility of the emergence of a drug-resistant subpopulation or of changes in the metabolic pathways of P. falciparum by the Trager-Jensen method and a chloroquine-resistant characteristic of the strain.

TL;DR: Using transmission and scanning electron microscopy, the early migration of juvenile Fasciola hepatica was examined in naive and resistant rats and eosinophils were seen degranulating onto the fluke surface, and this appeared to result in the erosion of the tegumental syncytium.

TL;DR: Histopathological studies showed that more than 40% of larvae obtained from immunized hogs were completely destroyed and the others were seen in various stages of degeneration.

TL;DR: The results obtained indicate that the surface of L. m.

TL;DR: The intact epicuticles of Strongyloides ratti stage-3 larvae and Trichinella spiralis stage-1 larvae were found to have a surface net negative charge and it is suggested that the association of highly sulfated mucopolysaccharides with the epicuticular surface of free-living nematodes such as S. ratti L3 may reflect a greater need to protect against surface desiccation.

TL;DR: The phenotypically suppressed HU-resistant gene was revealed by genetic crosses with wild type T. gondii, and the lack of doubly resistant recombinants was shown to be a consequence of phenotypic suppression of HU resistance by FUDR resistance.

TL;DR: The study of the intracellular and extracellular differentiation of Trypanosoma cruzi amastigotes suggests that ADP-ribosyl transferase frequently constitutes an important element in the mechanism of eukaryotic cell differentiation.

TL;DR: Schistosoma mansoni schistosomules prepared from cercariae by seven in vitro techniques had not all reached the same state of development at the end of the incubation period as scored by seven parameters: water tolerance, Cercarienhüllen Reaktion, and presence of the glycocalyx.

TL;DR: The finding of significant eosinophil accumulations at tick feeding sites of both hosts indicates that these cells may also contribute to acquired resistance to tick feeding.

TL;DR: Introduction of tick-resistant cattle provided a more effective means of reducing the risk of babesiosis outbreaks and the economic implications of computer-simulated babesia bovis control alternatives should be analyzed.

TL;DR: The antigenic constituents of a trichloroacetic acid-soluble fraction of adult Schistosoma japonicum were studied with immunoelectrophoresis, and compared with those of Schistsosoma mansoni, and most of the circulating antigens were shown to be gut-associated.

TL;DR: Difluoromethylornithine inhibits the sporogonous cycle of the malaria parasite Plasmodium berghei in the mosquito vector Anopheles stephensi and bites of mice subsequently bitten by mosquitoes treated with DFMO did not contract malaria.

TL;DR: The IELISA showed a 32-fold increase in specificity for the quantitative detection of specific antigenic determinants when monoclonal antibodies were used to restrict the heterogeneity of the measured response and the technique proved satisfactory for the quantification of parasitic burden in mice and the detection of active infections in humans.

TL;DR: Cultivation of fourth stage Brugia pahangi and B. malayi larvae from infective larvae were obtained in culture medium RPMI 1640 supplemented with 10% human AB serum and an LCC-MK2 rhesus monkey kidney continuous cell line feeder layer, and served as a source for collection of the worms' secretory, excretory, and moulting antigens.

TL;DR: Data demonstrate that the destruction of infectivity of T. spiralis is time-temperature related, and larvae rendered noninfective after 6 min at 55 C.

TL;DR: Short-term incubations in vitro of immature and adult Fasciola hepatica with DPT-FA demonstrated a time/dose-dependent, irreversible paralysis that involved an increase in muscular tension and decrease in contraction amplitude.

TL;DR: In hogs naturally infected with Taenia solium larvae (i.e., Cysticercus cellulosae), the host response induced by antigens obtained from the larvae was studied to show an intense inflammatory reaction surrounding the larvae.

TL;DR: Alterations in brain energy status and pH cannot account for cerebral signs or death in this proposed mouse model of cerebral malaria.

TL;DR: Heterosexual attraction was similar in designs using perforated chimneys and those with 50,000 and 12,000 Mr exclusions, but none was seen in chimney designs with 1000Mr exclusions.

TL;DR: It is shown here that Leishmania mexicana amazonensis, an agent of cutaneous disease, is growth restricted in vitro at 37.5 C and viable at 34 C, and this temperature sensitivity has been observed in both infected macrophage cultures and isolated amastigotes.