Journal ArticleDOI
A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.
TLDR
An improved method for gene replacement in Pseudomonas aeruginosa was developed and a cassette was constructed that contains a GmR selectable marker next to the green fluorescent protein structural gene, with both markers being flanked by Flp recombinase target (FRT) sites.About:
This article is published in Gene.The article was published on 1998-05-28. It has received 1858 citations till now. The article focuses on the topics: Aminodeoxychorismate lyase & Selectable marker.read more
Citations
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Journal ArticleDOI
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation
TL;DR: A rapid microcentrifuge-based method is described for preparation of Pseudomonas aeruginosa electrocompetent cells with up to 10,000-fold increased transformation efficiencies over existing procedures, which enables the use of transformation for all applications requiring DNA transfer.
Journal ArticleDOI
Active Starvation Responses Mediate Antibiotic Tolerance in Biofilms and Nutrient-Limited Bacteria
Dao Nguyen,Amruta Joshi-Datar,François Lépine,Elizabeth Bauerle,Oyebode Olakanmi,Karlyn D. Beer,Geoffrey McKay,Richard Siehnel,James Schafhauser,Yun Wang,Bradley E. Britigan,Bradley E. Britigan,Pradeep K. Singh +12 more
TL;DR: The experiments link SR-mediated tolerance to reduced levels of oxidant stress in bacterial cells, and inactivating this protective mechanism sensitized biofilms by several orders of magnitude to four different classes of antibiotics and markedly enhanced the efficacy of antibiotic treatment in experimental infections.
Journal ArticleDOI
A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels.
TL;DR: The data suggest that the wsp signal transduction pathway regulates biofilm formation through modulation of cyclic diguanylate levels.
Journal ArticleDOI
Iron and Pseudomonas aeruginosa biofilm formation
TL;DR: It is suggested that the functional iron signal for P. aeruginosa biofilm development is active transport of chelated iron or the level of internal iron, and Fur, the known Fur-controlled small regulatory RNAs, is involved in iron signaling.
Journal ArticleDOI
Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms
Lisa Friedman,Roberto Kolter +1 more
TL;DR: Results suggest that the pel genes are responsible for the production of a glucose‐rich matrix material required for the formation of biofilms by P. aeruginosa PA14.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
PatentDOI
FACS-optimized mutants of the green fluorescent protein (GFP)
TL;DR: In this article, three classes of GFP mutants having single excitation maxima around 488 nm are shown to be brighter than wild-type GFP following 488-nm excitation.
Book
A short course in bacterial genetics
TL;DR: This second edition of Molecular Cloning reflects more comprehensive coverage of topics previously included in the first edition as well as the addition of new chapters, such as those on oligonucleotide probes and mutagenesis, in vitro amplification by the polymerase chain reaction, expression of cloned genes in Escherichia coli and mammalian cells, and analysis of proteins ex pressed from cloning genes.
Journal ArticleDOI
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant.
TL;DR: Two cassettes with tetracycline-resistance (TcR) and kanamycin-res resistance (KmR) determinants have been developed for the construction of insertion and deletion mutants of cloned genes in Escherichia coli.
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