Showing papers in "Virus Research in 1999"

TL;DR: The presence of at least two potentially virulent EV71 co-circulating in Malaysia during an outbreak of hand, foot and mouth disease in Malaysia in 1997 is suggested.

TL;DR: VHSV has previously been reported from cod and herring, but not from the other five species, and a virus preliminary identified as iridovirus (lymphocystis virus) was isolated from seven samples of long rough dab Hippoglossoides platessoides.

TL;DR: Phylogenetic analysis of pol gene sequences resulted in groupings which correspond closely with the previously described groupings, including recent data which places the two avian coronaviruses—infectious bronchitis virus of chickens and turkey coronavirus

TL;DR: The cellular immune response to a European isolate of porcine reproductive and respiratory syndrome (PRRS) virus in animals recovered from the experimental infection has been studied in vitro and may play a relevant role in the clearance of PRRS virus and the recovery of the infection.

TL;DR: Results provide the first evidence that iridoviruses naturally infect animals belonging to different taxonomic classes, and strengthen the suggestion that fish may serve as a reservoir for amphibian viruses or vice versa.

TL;DR: A ribonuclease (RNase) protection assay has been used to detect nucleotide sequence variation within the nucleoprotein gene of 39 viral haemorrhagic septicaemia virus (VHSV) isolates of European marine origin and indicated that VHSV isolates recovered from wild caught fish around the British Isles were genetically related to isolate responsible for losses in farmed turbot.

TL;DR: The localization of the N protein in the absence of infection was studied by following fluorescence in MARC-145 cells transfected with a plasmid, which expressed the nucleocapsid protein fused to an enhanced green fluorescent protein (N-EGFP).

TL;DR: Porcine reproductive and respiratory syndrome virus (PRRSV), a recently discovered arterivirus swine pathogen, was shown to undergo homologous recombination, indicating that recombination is likely an important genetic mechanism contributing to PRRSV evolution.

TL;DR: This study demonstrates BPV gene expression in equine sarcoid tumours and provides the first evidence for a direct involvement of the virus in the pathogenesis of sarcoids.

TL;DR: Results suggesting that recombination and other rearrangements of the viral RNA genomes may be accomplished not only by the replicative template switching but also by nonreplicative mechanisms are reviewed.

TL;DR: It is confirmed that different genomic variants of Ad40 all carry an RGAD motif on the penton base, and found that the Ad41 prototype and several other genomic variant of Ad41 carry the motif IGDD in place of RGAD or RGD.

TL;DR: Results indicate that both juvenile and adult sea bass subject to mass mortality in Italy since summer 1995 are infected with a fish nodavirus and strongly suggest that the identified virus is the cause of the observed mortality.

TL;DR: The properties of the three defined proteolytic activities present in the picornaviruses are reviewed and the three-dimensional structures of the hepatitis A 3C proteinase and the leader proteinase of foot-and-mouth disease virus as well as a model of the structure of the HRV2 2A proteinase are compared with those of chymotrypsin, papain and streptomyces griseus A proteinase.

TL;DR: The analysis suggested that the overall structural integrity of the flaviviral 3'-UTR is essential for optimal performance of its promotor function, although two distinct parts can be defined: the most 3'-terminal structures and sequences which may be critical for the initiation of minus-strand RNA synthesis, and more proximal structures and Sequence that possibly function as enhancers of viral RNA replication.

TL;DR: Dissection of the diversity of viral and host-specific determinants in susceptible and resistant hosts will allow to define the protective mechanisms that mediate resistance to the development of life-threatening acute and chronic enterovirus myocarditis.

TL;DR: A virus isolated from soft-shelled turtle with 'red neck disease' on a farm in Shenzhen, China, the virus multiplied and caused a cytopathogenic effect (CPE) at 15-30 degrees C in CO, FHM, CK and BF-2 cells, indicating the presence of a DNA genome.

TL;DR: A vaccination trial including lower doses of DNA and different virus isolates was performed and cumulative mortalities demonstrated that even the lowest dose of DNA tested induced protective immunity against both virus isolate.

TL;DR: The biological activity of the genotypic variants SP2A, SP2B, and SP2C when compared in terms of LD50 and LT50, were not significantly different, however, the SP2D genotypes were found to be significantly less infective (higher LD50).

TL;DR: Phylogenetic analysis of the S10 gene segregated the Chinese viruses into a monophyletic group distinct from the US viruses, whereas similarAnalysis of the L2 gene segregated strains of BTV according to serotype, regardless of geographic origin.

TL;DR: The consensus nucleotide sequence of the entire genome of foot-and-mouth disease virus (FMDV) (biological clone C-S8c1) has been completed, and compared with that of two persistent derivatives R99 and R146, rescued after 99 and 146 passages of the carrier BHK-21 cells.

TL;DR: Equine herpesvirus 1 (EHV-1) strain RacL 11 mutant was constructed that carries the Escherichia coli LacZ gene instead of the open reading frame encoding glycoprotein C (gC) and plays important roles in the early steps of infection and in release of virions, especially in primary equine cells, and contributes to EHV- 1 virulence.

TL;DR: In addition to establishing diversity among reptilian paramyxoviruses, the phylogenetic groupings showed some correlation with geographic location, and clearly demonstrated a low level of host species-specificity within these viruses.

TL;DR: Each of the PB1, PB2, NA, HA, and M1 genes acquired gain-of-function mutations for mouse infection that involve structural motifs that may serve as markers for virulence or targets for antiviral therapy.

TL;DR: It is concluded that temperature plays an important role in GNNV infection and pathogenicity.

TL;DR: Analysis of 22 complete sequences of double-stranded DNA viruses reveals striking compositional asymmetries between leading and lagging, and between transcribed and non-transcribed strands, which switches polarity close to replication origins.

TL;DR: Similarities in both size and sequence of TCV and IBV N proteins and 3′ UTRs provide additional evidence that these avian coronaviruses are closely related.

TL;DR: The SHRV-G gene was successfully expressed in mammalian cells under the control of the cytomegalovirus (CMV) promoter, establishing that it can potentially be used in the production of pseudotyped retroviruses designed to infect fish.

TL;DR: Phylogenetic analysis indicates that the genotype of hantavirus endemic in Japanese C. rufocanus forms a distinct lineage within Puumala viruses.

TL;DR: The results showed that the sensitivity of nodavirus detection is greatly improved using a nested RT-PCR method and it was possible to monitor the presence of viral genome in asymptomatic carrier fish using this method.

TL;DR: It is shown that parvovirus H-1 infection induced early biochemical changes pointing to apoptotic events also in the transformed human keratinocyte cell line, HeLa, and the transformed rat fibroblastcell line, P1, suggesting NAD-consuming enzymes may promote necrosis in a direct way rather than through inducing the overall drop of intracellular NAD.