Journal ArticleDOI
15N-permethylated amino acids as efficient probes for MRI-DNP applications.
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TLDR
The obtained results make perdeuterated amino acids excellent candidates for innovative DNP (15)N-MRI applications such as perfusion or targeting studies.Abstract:
The synthesis, NMR properties and preliminary polarization tests on protonated and perdeuterated forms of α-trimethylglutamine (NMe3Gln), α-trimethylglutamate (NMe3Glu) and e-trimethyllysine (NMe3Lys) are reported. The (15)N-permethylated, perdeuterated amino acids display very long (15)N-T1 values, ranging between 190 and 330 s, are well polarized by the dynamic nuclear polarization (DNP) procedure, yielding good polarization levels (10%), and appear to be well tolerated by cells and mice. The obtained results make perdeuterated amino acids excellent candidates for innovative DNP (15)N-MRI applications such as perfusion or targeting studies.read more
Citations
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Journal ArticleDOI
Hyperpolarized NMR probes for biological assays.
TL;DR: A survey of strategies used for the selection, design and use of hyperpolarized NMR probes in biological assays is provided, and current limitations and developments are described.
Journal ArticleDOI
Hyperpolarization of Deuterated Metabolites via Remote Cross-Polarization and Dissolution Dynamic Nuclear Polarization
TL;DR: In this article, Hartmann-Hahn cross-polarization (CP) at low temperatures (1.2 K) with dissolution dynamic nuclear polarization (D-DNP) was used to enhance the nuclear spin polarization of 13C nuclei.
Journal Article
Hyperpolarization of Deuterated Metabolites via Remote Cross-Polarization and Dissolution Dynamic Nuclear Polarization B
TL;DR: Time scales over which metabolic processes and chemical reactions can be monitored are extended and polarization levels are increased and the lifetimes of the enhanced (13)C polarization during and after transfer from the polarizer to the NMR or MRI system are extended.
Journal ArticleDOI
NMR insights into the inner workings of living cells.
Journal ArticleDOI
Design of a 15 N Molecular Unit to Achieve Long Retention of Hyperpolarized Spin State
Hiroshi Nonaka,Masashi Hirano,Yuki Imakura,Yoichi Takakusagi,Kazuhiro Ichikawa,Shinsuke Sando +5 more
TL;DR: It is reported that [15N, D14]trimethylphenylammonium (TMPA) has a remarkably long spin–lattice relaxation time and achieves a long retention of the hyperpolarized state.
References
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Journal ArticleDOI
Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR
Jan Henrik Ardenkjær-Larsen,Björn Fridlund,Andreas Gram,Georg Hansson,Lennart Hansson,Mathilde H. Lerche,Rolf Servin,Mikkel Thaning,Klaes Golman +8 more
TL;DR: The method can be used generally for signal enhancement and reduction of measurement time in liquid-state NMR and opens up for a variety of in vitro and in vivo applications of DNP-enhanced NMR.
Journal ArticleDOI
Principles of dynamic nuclear polarisation
Anatole Abragam,M Goldman +1 more
TL;DR: In this paper, a survey of the theories of spin temperature and nuclear relaxation in solids is presented, followed by a short section on the so-called well-resolved solid effect, and a much more detailed one on DNP by thermal mixing in the non-linear low spin-temperature domain.
Journal ArticleDOI
Production of hyperpolarized [1,4-13C2]malate from [1,4-13C2]fumarate is a marker of cell necrosis and treatment response in tumors
Ferdia A. Gallagher,Mikko I. Kettunen,De-En Hu,Pernille Rose Jensen,René in ‘t Zandt,Magnus Karlsson,Anna Gisselsson,Sarah K. Nelson,Timothy H. Witney,Sarah E. Bohndiek,Georg Hansson,Torben Peitersen,Mathilde H. Lerche,Kevin M. Brindle +13 more
TL;DR: The formation of hyperpolarized 13C-labeled malate from [1,4-13C2]fumarate appears to be a sensitive marker of tumor cell death in vivo and could be used to detect the early response of tumors to treatment.
Journal ArticleDOI
Tumor imaging using hyperpolarized 13C magnetic resonance spectroscopy
TL;DR: This review highlights the most significant molecules investigated to date in preclinical cancer models, either in terms of their demonstrated metabolism in vivo or the biological processes that they can probe, and techniques to image these molecules are discussed.
Journal ArticleDOI
Long-lived states to sustain hyperpolarized magnetization
Paul R. Vasos,Riddhiman Sarkar,Puneet Ahuja,Sami Jannin,Jean-Philippe Ansermet,J. A. Konter,Patrick Hautle,B. van den Brandt,Geoffrey Bodenhausen +8 more
TL;DR: A method to preserve enhanced (“hyperpolarized”) magnetization by conversion into long-lived states (LLS) is designed and it is shown that these enhanced long- lived states can be generated for proton spins, which afford sensitive detection.
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