Book ChapterDOI
[27] The cyanogen bromide reaction
TLDR
The reaction is applied in a number of ways, such as in the structural elucidation of peptides and proteins, the detection of multiple forms of enzymes and of oxidized residues of methionine, the preparation of physiologically active peptide fragments, the location of newly introduced cross-linkages in enzymes, and the identification and characterization of fragments from enzyme active sites.Abstract:
Publisher Summary This chapter discusses the applications of the cyanogens bromide reaction Cyanogen bromide is capable of cleaving thioethers The action of cyanogen bromide upon proteins is unique in its selective attack on methionine The reaction of methionine with cyanogen bromide is greatly facilitated by the strong neighboring group effect exerted by the carboxyl group Cyanogen bromide is synthesized from bromine and potassium cyanide The selectivity of the reaction of cyanogen bromide with amino acids depends on pH The selectivity of the cyanogen bromide reaction is demonstrated by exposure to cyanogen bromide of a standard mixture of amino acids, as is used for calibration purposes in automated amino acid analyzer systems A number of applications to the general structural elucidation of peptides and proteins are: ribonuclease, rabbit γ-globulin, and chymotrypsin The reaction is applied in many other instances and is useful in a number of ways, such as in the structural elucidation of peptides and proteins, the detection of multiple forms of enzymes and of oxidized residues of methionine, the preparation of physiologically active peptide fragments, the location of newly introduced cross-linkages in enzymes,and the identification and characterization of fragments from enzyme active sitesread more
Citations
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The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.
E Rinderknecht,R E Humbel +1 more
TL;DR: The number of differences in amino acid positions between IGF-I and insulins suggests that duplication of the gene of the common ancestor of proinsulin and IGF occurred before the time of appearance of the vertebrates.
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A cellular gene encodes scrapie PrP 27-30 protein
Bruno Oesch,Bruno Oesch,David Westaway,Monika Wälchli,Monika Wälchli,Michael P. McKinley,Stephen B. H. Kent,Stephen B. H. Kent,Ruedi Aebersold,Ruedi Aebersold,Ronald A. Barry,Paul Tempst,Paul Tempst,David B. Teplow,David B. Teplow,Leroy Hood,Leroy Hood,Stanley B. Prusiner,Stanley B. Prusiner,Charles Weissmann,Charles Weissmann +20 more
TL;DR: A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein.
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Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-hANP)
Kenji Kangawa,Hisayuki Matsuo +1 more
TL;DR: A survey for natriuretic factors in human atrial extract was performed by using in vitro assay for the relaxant effect on the contractility of chick rectum.
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Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin
Keiichi Itakura,Tadaaki Hirose,Roberto Crea,Arthur D. Riggs,Herbert L. Heyneker,Francisco Bolívar,Herbert W. Boyer +6 more
TL;DR: This work has reported the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin, including the sequence of amino acids corresponding to somatostatin, in vitro.
References
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Automatic recording apparatus for use in the chromatography of amino acids.
Journal ArticleDOI
Dithiothreitol, a New Protective Reagent for SH Groups*
TL;DR: Strominger, J. L. H. (1960), Instruction Manual and Handbook, Beckman/Spinco Model 120 Amino Acid Analyzer, Palo Alto, California,Beckman Instruments Inc., Spinco Division.
Journal ArticleDOI
Gel filtration: a method for desalting and group separation.
Jerker Porath,Per Flodin +1 more
TL;DR: The method is based on a column procedure similar to chromatography in which the stationary phase is comprised of a new type of gel which consists of hydrophilic chains which are cross-linked.
Journal ArticleDOI
Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds.
TL;DR: The results indicate that the proteolytic enzyme splits off an inactive portion of the molecule; and that subsequent to this reaction the bivalent residue can be divided into two univalent fragments by breaking the disulfide bonds which hold them together.
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