A New Functional Role of the Fibrinogen RGD Motif as the Molecular Switch That Selectively Triggers Integrin αIIbβ3-dependent RhoA Activation during Cell Spreading
Alexandre Salsmann,Elisabeth Schaffner-Reckinger,Fabrice Kabile,Sébastien Plançon,Nelly Kieffer +4 more
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TLDR
The data provide evidence that the γ400-411 site by itself is able to initiate αIIbβ3 clustering and recruitment of intracellular proteins to early focal complexes, mediating cell attachment, FAK phosphorylation, and Rac1 activation, while the RGD motif subsequently acts as a molecular switch on the β3 subunit to trigger cell spreading.About:
This article is published in Journal of Biological Chemistry.The article was published on 2005-09-30 and is currently open access. It has received 54 citations till now. The article focuses on the topics: RGD motif & Fibrinogen binding.read more
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Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers
TL;DR: The effect of surface wettability on fibrinogen adsorption, platelet adhesion and platelet activation was investigated using self-assembled monolayers containing different ratios of longer chain methyl- and shorter chain hydroxyl-terminated alkanethiols (C15CH3 vs. C11OH) on gold.
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Reactive oxygen species inhibit adhesion of mesenchymal stem cells implanted into ischemic myocardium via interference of focal adhesion complex.
Heesang Song,Min Ji Cha,Byeong-Wook Song,Il-Kwon Kim,Woochul Chang,Soyeon Lim,Eun-Ju Choi,Onju Ham,Se-Yeon Lee,Namsik Chung,Yangsoo Jang,Ki-Chul Hwang +11 more
TL;DR: Investigation of the role of ROS on MSC adhesion indicates that ROS inhibit cellular adhesion of engrafted MSCs and provides evidence that the elimination of ROS might be a novel strategy for improving the survival ofEngrafted M SCs.
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Integrin-directed modulation of macrophage responses to biomaterials.
Toral Zaveri,Jamal S. Lewis,Natalia V. Dolgova,Michael J. Clare-Salzler,Benjamin G. Keselowsky +4 more
TL;DR: Findings indicate integrin Mac-1 and RGD-binding integrins are involved and may serve as therapeutic targets to mitigate macrophage inflammatory responses to both particulate and bulk biomaterials.
Journal ArticleDOI
Biomimetic Architectures for Peripheral Nerve Repair: A Review of Biofabrication Strategies.
Paul Wieringa,Ana Rita Gonçalves de Pinho,Silvestro Micera,Silvestro Micera,Richard J. A. van Wezel,Richard J. A. van Wezel,Lorenzo Moroni +6 more
TL;DR: A roadmap of past, present, and emerging fabrication techniques to inform and motivate new developments in the field of peripheral nerve regeneration is outlined, placing emphasis on approaches that create structural cues within a hollow NGC lumen in order to match or exceed the regenerative performance of the autograft.
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A nonsynonymous SNP in the ITGB3 gene disrupts the conserved membrane-proximal cytoplasmic salt bridge in the αIIbβ3 integrin and cosegregates dominantly with abnormal proplatelet formation and macrothrombocytopenia
Cedric Ghevaert,Cedric Ghevaert,Alexandre Salsmann,Nicholas A. Watkins,Nicholas A. Watkins,Elisabeth Schaffner-Reckinger,Angela Rankin,Stephen F. Garner,Stephen F. Garner,Jonathan Stephens,Graham A. Smith,Najet Debili,William Vainchenker,Philip G. de Groot,James A. Huntington,Michael Laffan,Nelly Kieffer,Willem H. Ouwehand,Willem H. Ouwehand +18 more
TL;DR: It is concluded that the constitutive activation of the alphaIIbbeta3-H723 receptor causes abnormal proplatelet formation, leading to incorrect sizing of platelets and the thrombocytopenia observed in the pedigree.
References
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Rho GTPases and the Actin Cytoskeleton
TL;DR: Members of the Rho family of small guanosine triphosphatases have emerged as key regulators of the actin cytoskeleton, and through their interaction with multiple target proteins, they ensure coordinated control of other cellular activities such as gene transcription and adhesion.
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Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
Catherine D. Nobes,Alan Hall +1 more
TL;DR: It is reported here that cdc42, another member of the rho family, triggers the formation of a third type of actin-based structure found at the cell periphery, filopodia, in addition to stress fibers, and rho controls the assembly of focal adhesion complexes.
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Crystal Structure of the Extracellular Segment of Integrin αVβ3 in Complex with an Arg-Gly-Asp Ligand
Jian-Ping Xiong,Thilo Stehle,Rongguang Zhang,Andrzej Joachimiak,Matthias Frech,Simon L. Goodman,M. Amin Arnaout +6 more
TL;DR: The crystal structure of the extracellular segment of integrin αVβ3 in complex with a cyclic peptide presenting the Arg-Gly-Asp sequence is reported and ligand binding induces small changes in the orientation of αV relative to β3.
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Regulation of the small GTP‐binding protein Rho by cell adhesion and the cytoskeleton
TL;DR: The results show that ECM, cytoskeletal structures and soluble factors all contribute to regulation of Rho activity, and both cytochalasin D and colchicine trigger Rho activation despite their opposite effects on stress fibers and focal adhesions.
Journal ArticleDOI
Ligand binding to integrins.
TL;DR: The number of integrins and the remarkable breadth of their cellular distribution support the statement that the phenotype of virtually every cell is uniquely influenced by its display ofintegrins.