Journal ArticleDOI
A ras-related gene with transformation suppressor activity
Reads0
Chats0
TLDR
The Krev-1 gene seems to play an important role(s) in a wide variety of tissues, and may be involved in the negative growth regulation of certain cell types.Citations
More filters
Journal ArticleDOI
Mechanisms of type-I- and type-II-interferon-mediated signalling.
TL;DR: It is anticipated that an increased understanding of the contributions of these recently identified pathways will advance current thinking about how interferons work.
Journal ArticleDOI
Small GTP-Binding Proteins
TL;DR: In this review, functions of small G proteins and their modes of activation and action are described.
Journal ArticleDOI
The GTPase superfamily: a conserved switch for diverse cell functions
TL;DR: A molecular switch is a molecular switch whose "on" and "off" states are triggered by binding and hydrolysis of GTP as discussed by the authors. But the mechanism in myriad versions of the switch can be traced back to a single primordial protein.
Journal ArticleDOI
Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP
J. de Rooij,Fried J. T. Zwartkruis,M. H. G. Verheijen,Robbert H. Cool,Sebastian M.B. Nijman,Alfred Wittinghofer,Johannes L. Bos +6 more
TL;DR: The cloned gene encoding a guanine-nucleotide-exchange factor (GEF) for Rap1 is named Epac, which contains a cAMP-binding site and a domain that is homologous to domains of known GEFs for Ras and Rap1 that is regulated directly by cAMP.
Journal ArticleDOI
Molecular themes in oncogenesis
TL;DR: It seems likely that most malignancies arise from the collaborative effects of dominant and recessive lesions, andumeration of the number of tumor suppressor genes afflicted in any given tumor may be greater.
References
More filters
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
High-efficiency transformation of mammalian cells by plasmid DNA.
TL;DR: A simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells are described and linear DNA is almost inactive in mammalian cells.
Journal ArticleDOI
Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes
Don W. Cleveland,Margaret A. Lopata,Raymond J. MacDonald,Nicholas J. Cowan,William J. Rutter,Marc W. Kirschner +5 more
TL;DR: Bacterial clones containing inserted DNA sequences specific for α- Tubulin, β-tubulin,β-Actin and γ-actin have been constructed from mRNA of embryonic chick brain and are able to hybridize under stringent conditions to DNA of all vertebrates tested, as well as to sea urchin DNA, but not to yeast DNA.
Journal ArticleDOI
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells
TL;DR: The feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase to the aprt+ phenotype by means of DNA-mediated gene transfer is demonstrated.
Journal ArticleDOI
Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue
TL;DR: DNA sequence analysis of the activated oncogene from the T24 human bladder carcinoma line and two alleles of its normal cellular progenitor (c-Ha-ras-1) indicates that the genes encompass at least four exons.