A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis
Chanjuan Mao,Lu Songchong,Bo Lv,Bin Zhang,Jiabin Shen,He Jianmei,Liqiong Luo,Dandan Xi,Xu Chen,Feng Ming +9 more
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TLDR
A rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence and elucidates the transcriptional network of ABA production during leaf senesence in rice is demonstrated.Abstract:
It is well known that abscisic acid (ABA)-induced leaf senescence and premature leaf senescence negatively affect the yield of rice (Oryza sativa). However, the molecular mechanism underlying this relationship, especially the upstream transcriptional network that modulates ABA level during leaf senescence, remains largely unknown. Here, we demonstrate a rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence. Overexpression of OsNAC2 dramatically accelerated leaf senescence, whereas its knockdown lines showed a delay in leaf senescence. Chromatin immunoprecipitation-quantitative PCR, dual-luciferase, and yeast one-hybrid assays demonstrated that OsNAC2 directly activates expression of chlorophyll degradation genes, OsSGR and OsNYC3. Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly up-regulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as down-regulating the ABA catabolic gene (OsABA8ox1). Interestingly, OsNAC2 is upregulated by a lower level of ABA but downregulated by a higher level of ABA, indicating a feedback repression of OsNAC2 by ABA. Additionally, reduced OsNAC2 expression leads to about 10% increase in the grain yield of RNAi lines. The novel ABA-NAC-SAGs regulatory module might provide a new insight into the molecular action of ABA to enhance leaf senescence and elucidates the transcriptional network of ABA production during leaf senescence in rice.read more
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NAC transcription factor TgNAP promotes tulip petal senescence.
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Zohreh Hajibarat,Abbas Saidi +1 more
TL;DR: In this article , the role of nitrogen transporters, transcription factors and cell wall degradation enzymes related to senescence during drought stress were surveyed and discussed the regulatory pathways of genes as a result of the degradation of proteins during the leaf aging process and the relevant evidence for the balance between grain filling and protein breakdown during grain filling in cereals is presented.
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Identification and characterization of miRNAs during flag leaf senescence in rice by high-throughput sequencing
Jyothish Madambikattil Sasi,Cheeni Vijaya Kumar,Balaji Mani,Ankur R. Bhardwaj,Manu Agarwal,Surekha Katiyar-Agarwal +5 more
TL;DR: A repository of senescence-related miRNAs is generated that can be utilized to contemplate molecular approaches for manipulating the timing of flag leafsenescence in rice and other related crops.
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Physiological and transcriptional responses of Alternaria alternata induced abnormal leaf senescence in Pyrus pyrifolia
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Transcriptome-wide identification, classification, and characterization of NAC family genes in Bamboo Bambusa emeiensis
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References
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