A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis
Chanjuan Mao,Lu Songchong,Bo Lv,Bin Zhang,Jiabin Shen,He Jianmei,Liqiong Luo,Dandan Xi,Xu Chen,Feng Ming +9 more
Reads0
Chats0
TLDR
A rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence and elucidates the transcriptional network of ABA production during leaf senesence in rice is demonstrated.Abstract:
It is well known that abscisic acid (ABA)-induced leaf senescence and premature leaf senescence negatively affect the yield of rice (Oryza sativa). However, the molecular mechanism underlying this relationship, especially the upstream transcriptional network that modulates ABA level during leaf senescence, remains largely unknown. Here, we demonstrate a rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence. Overexpression of OsNAC2 dramatically accelerated leaf senescence, whereas its knockdown lines showed a delay in leaf senescence. Chromatin immunoprecipitation-quantitative PCR, dual-luciferase, and yeast one-hybrid assays demonstrated that OsNAC2 directly activates expression of chlorophyll degradation genes, OsSGR and OsNYC3. Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly up-regulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as down-regulating the ABA catabolic gene (OsABA8ox1). Interestingly, OsNAC2 is upregulated by a lower level of ABA but downregulated by a higher level of ABA, indicating a feedback repression of OsNAC2 by ABA. Additionally, reduced OsNAC2 expression leads to about 10% increase in the grain yield of RNAi lines. The novel ABA-NAC-SAGs regulatory module might provide a new insight into the molecular action of ABA to enhance leaf senescence and elucidates the transcriptional network of ABA production during leaf senescence in rice.read more
Citations
More filters
Posted ContentDOI
miR2105 regulates ABA biosynthesis via OsbZIP86-OsNCED3 module to contribute to drought tolerance in rice
Weiwei Gao,Mingkang Li,Songguang Yang,Chunzhi Gao,Su Yan,Xuan Zeng,Zhengli Jiao,Weijuan Xu,Mingyong Zhang,Kuaifei Xia +9 more
TL;DR: In this article, the authors identified a rice miRNA, osa-miR2105, which plays a crucial role in induced abscisic acid (ABA) biosynthesis under drought stress.
Journal ArticleDOI
Physiological and morphological factors affecting leaf sheath reinforcement and their contribution to lodging resistance in rice
TL;DR: In this article , the morphological and physiological traits that are presumed to be related to leaf sheath reinforcement were examined in 'Chugoku 117' and 'Koshihikari', rice varieties with high and low degrees of leafsheath reinforcement, respectively.
Journal ArticleDOI
Rice sl-MH-1 mutant induces cell death and confers blast resistance via the synergistic roles of signaling systems
Dagang Tian,Yan Lin,Shengping Li,Yiyang Cao,Gang Li,Xinrui Guo,Ziqiang Chen,Zai-jie Chen,Feng Wang,Zonghua Wang +9 more
TL;DR: In this paper , the authors performed a multi-omics analysis to clarify the fundamental mechanisms at protein, gene transcript, and metabolite levels, and found that cell death and Magnaporthe oryzae infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid (SA), jasmonic acid (JA), and abscisic acid(ABA) in multiple regulatory layers.
Journal ArticleDOI
1-Methylcyclopropene retards pak choi (Brassica rapa subsp. chinensis) yellowing via BcNAC055-, BcMYB44- and BcOBF1-mediated regulation of the key chlorophyll degrading gene BcNYC1 during storage at 20 °C
TL;DR: In this article , 1-MCP fumigation alleviated the yellowing process of pak choi as proved by the maintenance of higher level of colour, chlorophyll content and appearance.
Journal ArticleDOI
Functional Characterization of the CpNAC1 Promoter and Gene from Chimonanthus praecox in Arabidopsis
Xiaoyang Zhao,Jiahui Zhao,Qing Yang,Min Huang,Yang Song,Mingyang Li,Shunzhao Sui,Daofeng Liu +7 more
TL;DR: In this article , the CpNAC1 promoter and gene from wintersweet were localized to the nucleus and showed transcriptional activation activity in almost all tissues tested, with the highest levels found in mature leaves and flower buds.
References
More filters
Journal ArticleDOI
Abscisic Acid: Emergence of a Core Signaling Network
TL;DR: A new model for ABA action has been proposed and validated, in which the soluble PYR/PYL/RCAR receptors function at the apex of a negative regulatory pathway to directly regulate PP2C phosphatases, which in turn directly regulate SnRK2 kinases.
Journal ArticleDOI
Abscisic acid biosynthesis and catabolism
Eiji Nambara,Annie Marion-Poll +1 more
TL;DR: Identification of ABA metabolic genes has revealed that multiple metabolic steps are differentially regulated to fine-tune the ABA level at both transcriptional and post-transcriptional levels.
Journal ArticleDOI
Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants
Roger P. Hellens,Andrew C. Allan,Ellen N. Friel,Karen Bolitho,Karryn Grafton,Matthew D. Templeton,Sakuntala Karunairetnam,Andrew P. Gleave,William A. Laing +8 more
TL;DR: In this article, a series of plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves, are described and compared to conventional binary vectors for stable transformation such as transformation selection genes.
Journal ArticleDOI
NAC transcription factors: structurally distinct, functionally diverse
TL;DR: The biological and molecular functions of the NAC family are summarized, paying particular attention to the intricate regulation of NAC protein level and localization, and to the first indications of Nac participation in transcription factor networks.
Journal ArticleDOI
Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression.
Hiroshi Abe,Kazuko Yamaguchi-Shinozaki,Takeshi Urao,Toshisuke Iwasaki,Daijiro Hosokawa,Kazuo Shinozaki +5 more
TL;DR: Results indicate that both the rd22BP1 (MYC) and ATMYB2 (MYB) proteins function as transcriptional activators in the dehydration- and ABA-inducible expression of the r d22 gene.
Related Papers (5)
AtNAP, a NAC family transcription factor, has an important role in leaf senescence
Yongfeng Guo,Susheng Gan +1 more
High-Resolution Temporal Profiling of Transcripts during Arabidopsis Leaf Senescence Reveals a Distinct Chronology of Processes and Regulation
Emily Breeze,Elizabeth Harrison,Stuart McHattie,Linda Karen Hughes,Richard Hickman,Claire Hill,Steven J. Kiddle,Youn-Sung Kim,Christopher A. Penfold,Dafyd J. Jenkins,Cunjin Zhang,Karl Morris,Carol E. Jenner,Stephen D. Jackson,Brian Thomas,Alex Tabrett,Roxane Legaie,Jonathan D. Moore,David L. Wild,Sascha Ott,David A. Rand,Jim Beynon,Katherine J. Denby,Andrew Mead,Vicky Buchanan-Wollaston +24 more