A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis
Chanjuan Mao,Lu Songchong,Bo Lv,Bin Zhang,Jiabin Shen,He Jianmei,Liqiong Luo,Dandan Xi,Xu Chen,Feng Ming +9 more
Reads0
Chats0
TLDR
A rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence and elucidates the transcriptional network of ABA production during leaf senesence in rice is demonstrated.Abstract:
It is well known that abscisic acid (ABA)-induced leaf senescence and premature leaf senescence negatively affect the yield of rice (Oryza sativa). However, the molecular mechanism underlying this relationship, especially the upstream transcriptional network that modulates ABA level during leaf senescence, remains largely unknown. Here, we demonstrate a rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence. Overexpression of OsNAC2 dramatically accelerated leaf senescence, whereas its knockdown lines showed a delay in leaf senescence. Chromatin immunoprecipitation-quantitative PCR, dual-luciferase, and yeast one-hybrid assays demonstrated that OsNAC2 directly activates expression of chlorophyll degradation genes, OsSGR and OsNYC3. Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly up-regulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as down-regulating the ABA catabolic gene (OsABA8ox1). Interestingly, OsNAC2 is upregulated by a lower level of ABA but downregulated by a higher level of ABA, indicating a feedback repression of OsNAC2 by ABA. Additionally, reduced OsNAC2 expression leads to about 10% increase in the grain yield of RNAi lines. The novel ABA-NAC-SAGs regulatory module might provide a new insight into the molecular action of ABA to enhance leaf senescence and elucidates the transcriptional network of ABA production during leaf senescence in rice.read more
Citations
More filters
Journal ArticleDOI
Integration of small RNA, degradome and proteome sequencing in Oryza sativa reveals a delayed senescence network in tetraploid rice seed
Huang Baosheng,Lu Gan,Chen Dongjie,Zhang Yachun,Yujie Zhang,Liu Xiangli,Chen Si,Zhisong Wei,Liqi Tong,Zhaojian Song,Zhang Xianhua,Detian Cai,Zhang Changfeng,Yuchi He +13 more
TL;DR: High-throughput sequencing was applied to systematically investigate variations in small RNAs, the degradome, and the proteome between tetraploid and diploid rice seeds, and showed that antioxidant enzymes were up-regulated in tetraPloid Rice seeds, relative to diploids.
Journal ArticleDOI
TaCKX2.2 Genes Coordinate Expression of Other TaCKX Family Members, Regulate Phytohormone Content and Yield-Related Traits of Wheat
Bartosz Jablonski,Karolina Szala,Mateusz Przyborowski,Andrzej Bajguz,Magdalena Chmur,Sebastian Gasparis,Wacław Orczyk,Anna Nadolska-Orczyk +7 more
Abstract: TaCKX gene family members (GFMs) play essential roles in the regulation of cytokinin during wheat development and significantly influence yield-related traits. However, detailed function of most of them is not known. To characterize the role of TaCKX2.2 genes we silenced all homoeologous copies of both TaCKX2.2.1 and TaCKX2.2.2 by RNAi technology and observed the effect of silencing in 7 DAP spikes of T1 and T2 generations. The levels of gene silencing of these developmentally regulated genes were different in both generations, which variously determined particular phenotypes. High silencing of TaCKX2.2.2 in T2 was accompanied by slight down-regulation of TaCKX2.2.1 and strong up-regulation of TaCKX5 and TaCKX11, and expression of TaCKX1, TaCKX2.1, and TaCKX9 was comparable to the non-silenced control. Co-ordinated expression of TaCKX2.2.2 with other TaCKX GFMs influenced phytohormonal homeostasis. Contents of isoprenoid, active cytokinins, their conjugates, and auxin in seven DAP spikes of silenced T2 plants increased from 1.27 to 2.51 times. However, benzyladenine (BA) and abscisic acid (ABA) contents were significantly reduced and GA3 was not detected. We documented a significant role of TaCKX2.2.2 in the regulation of thousand grain weight (TGW), grain number, and chlorophyll content, and demonstrated the formation of a homeostatic feedback loop between the transcription of tested genes and phytohormones. We also discuss the mechanism of regulation of yield-related traits.
Journal ArticleDOI
Enhancement of Nicotiana tabacum Resistance Against Dehydration-Induced Leaf Senescence via Metabolite/Phytohormone-Gene Regulatory Networks Modulated by Melatonin.
TL;DR: In this paper, the authors found that foliar spraying of melatonin markedly ameliorated dehydration-induced leaf senescence in Nicotiana tabacum, accompanied by attenuated oxidative damage, expression of senence-related genes, and reduced endogenous ABA production.
Journal ArticleDOI
Metabolism of abscisic acid in two contrasting rice genotypes submitted to recurrent water deficit.
Priscila Ariane Auler,Marcelo Nogueira do Amaral,Tatiana Rossatto,Rosane Lopez Crizel,Cristini Milech,Fábio Clasen Chaves,Gustavo Maia Souza,Eugenia Jacira Bolacel Braga +7 more
TL;DR: The results indicate that one of the factors that can promote greater tolerance for the upland genotype is the tradeoff between ABA and ABA-GE when plants are subjected to water deficits, and indicate that abscisic acid metabolism is altered due to the genotype.
Journal ArticleDOI
OUP accepted manuscript
TL;DR: In this article , the authors found that miR2105-directed cleavage of the OsbZIP86 mRNA can regulate the rice basic region-leucine zipper transcription factor (BZIP TF) at posttranscriptional and posttranslational levels to control drought-induced ABA biosynthesis via modulation of rice 9-cis-epoxycarotenoid dioxygenase (OsNCED3) expression.
References
More filters
Journal ArticleDOI
Abscisic Acid: Emergence of a Core Signaling Network
TL;DR: A new model for ABA action has been proposed and validated, in which the soluble PYR/PYL/RCAR receptors function at the apex of a negative regulatory pathway to directly regulate PP2C phosphatases, which in turn directly regulate SnRK2 kinases.
Journal ArticleDOI
Abscisic acid biosynthesis and catabolism
Eiji Nambara,Annie Marion-Poll +1 more
TL;DR: Identification of ABA metabolic genes has revealed that multiple metabolic steps are differentially regulated to fine-tune the ABA level at both transcriptional and post-transcriptional levels.
Journal ArticleDOI
Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants
Roger P. Hellens,Andrew C. Allan,Ellen N. Friel,Karen Bolitho,Karryn Grafton,Matthew D. Templeton,Sakuntala Karunairetnam,Andrew P. Gleave,William A. Laing +8 more
TL;DR: In this article, a series of plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves, are described and compared to conventional binary vectors for stable transformation such as transformation selection genes.
Journal ArticleDOI
NAC transcription factors: structurally distinct, functionally diverse
TL;DR: The biological and molecular functions of the NAC family are summarized, paying particular attention to the intricate regulation of NAC protein level and localization, and to the first indications of Nac participation in transcription factor networks.
Journal ArticleDOI
Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression.
Hiroshi Abe,Kazuko Yamaguchi-Shinozaki,Takeshi Urao,Toshisuke Iwasaki,Daijiro Hosokawa,Kazuo Shinozaki +5 more
TL;DR: Results indicate that both the rd22BP1 (MYC) and ATMYB2 (MYB) proteins function as transcriptional activators in the dehydration- and ABA-inducible expression of the r d22 gene.
Related Papers (5)
AtNAP, a NAC family transcription factor, has an important role in leaf senescence
Yongfeng Guo,Susheng Gan +1 more
High-Resolution Temporal Profiling of Transcripts during Arabidopsis Leaf Senescence Reveals a Distinct Chronology of Processes and Regulation
Emily Breeze,Elizabeth Harrison,Stuart McHattie,Linda Karen Hughes,Richard Hickman,Claire Hill,Steven J. Kiddle,Youn-Sung Kim,Christopher A. Penfold,Dafyd J. Jenkins,Cunjin Zhang,Karl Morris,Carol E. Jenner,Stephen D. Jackson,Brian Thomas,Alex Tabrett,Roxane Legaie,Jonathan D. Moore,David L. Wild,Sascha Ott,David A. Rand,Jim Beynon,Katherine J. Denby,Andrew Mead,Vicky Buchanan-Wollaston +24 more