A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis
Chanjuan Mao,Lu Songchong,Bo Lv,Bin Zhang,Jiabin Shen,He Jianmei,Liqiong Luo,Dandan Xi,Xu Chen,Feng Ming +9 more
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TLDR
A rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence and elucidates the transcriptional network of ABA production during leaf senesence in rice is demonstrated.Abstract:
It is well known that abscisic acid (ABA)-induced leaf senescence and premature leaf senescence negatively affect the yield of rice (Oryza sativa). However, the molecular mechanism underlying this relationship, especially the upstream transcriptional network that modulates ABA level during leaf senescence, remains largely unknown. Here, we demonstrate a rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence. Overexpression of OsNAC2 dramatically accelerated leaf senescence, whereas its knockdown lines showed a delay in leaf senescence. Chromatin immunoprecipitation-quantitative PCR, dual-luciferase, and yeast one-hybrid assays demonstrated that OsNAC2 directly activates expression of chlorophyll degradation genes, OsSGR and OsNYC3. Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly up-regulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as down-regulating the ABA catabolic gene (OsABA8ox1). Interestingly, OsNAC2 is upregulated by a lower level of ABA but downregulated by a higher level of ABA, indicating a feedback repression of OsNAC2 by ABA. Additionally, reduced OsNAC2 expression leads to about 10% increase in the grain yield of RNAi lines. The novel ABA-NAC-SAGs regulatory module might provide a new insight into the molecular action of ABA to enhance leaf senescence and elucidates the transcriptional network of ABA production during leaf senescence in rice.read more
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A Novel Protein RLS1 with NB-ARM Domains Is Involved in Chloroplast Degradation during Leaf Senescence in Rice
TL;DR: This study identified a previously uncharacterized NB-ARM protein involved in PCD during plant growth and development, providing a unique tool for dissecting possible autophagy-mediatedPCD during senescence in plants.
Journal ArticleDOI
OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling.
TL;DR: In this article, a rice NAC (NAM-ATAF-CUC) transcription factor, OsNAC2, was shown to increase the sensitivity of rice roots to ethylene during the seedling period.
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OsWRKY53 Promotes Abscisic Acid Accumulation to Accelerate Leaf Senescence and Inhibit Seed Germination by Downregulating Abscisic Acid Catabolic Genes in Rice
TL;DR: In this paper , the authors showed that OsWRKY53 is a positive regulator of leaf senescence and a negative regulator of seed germination in rice, and showed that the expression of OsWRky53 expression was induced in leaves under aging, dark, and ABA treatment.
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A study of leaf-senescence genes in rice based on a combination of genomics, proteomics and bioinformatics.
TL;DR: This study summarized recent reports on 125 rice leaf-senescence genes, providing an overview of the research progress in this field by analyzing the subcellular localizations, molecular functions and the relationship of them, and showed that chlorophyll synthesis and degradation, chloroplast development, abscisic acid pathway, jasmonic Acid pathway, nitrogen assimilation and ROS play an important role in regulating the leaf senescence in rice.
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Formyl tetrahydrofolate deformylase affects hydrogen peroxide accumulation and leaf senescence by regulating the folate status and redox homeostasis in rice
Xiong Erhui,Xiong Erhui,Guojun Dong,Fei Chen,Chen Zhang,Shan Li,Yanli Zhang,Jahidul Islam Shohag,Xiaoe Yang,Yihua Zhou,Qian Qian,Limin Wu,Yanchun Yu +12 more
TL;DR: The findings suggest that the accumulation of H2O2 in hpa1 may be mediated by an altered folate status and redox homeostasis, subsequently triggering leaf senescence.
References
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Abscisic Acid: Emergence of a Core Signaling Network
TL;DR: A new model for ABA action has been proposed and validated, in which the soluble PYR/PYL/RCAR receptors function at the apex of a negative regulatory pathway to directly regulate PP2C phosphatases, which in turn directly regulate SnRK2 kinases.
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Eiji Nambara,Annie Marion-Poll +1 more
TL;DR: Identification of ABA metabolic genes has revealed that multiple metabolic steps are differentially regulated to fine-tune the ABA level at both transcriptional and post-transcriptional levels.
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Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants
Roger P. Hellens,Andrew C. Allan,Ellen N. Friel,Karen Bolitho,Karryn Grafton,Matthew D. Templeton,Sakuntala Karunairetnam,Andrew P. Gleave,William A. Laing +8 more
TL;DR: In this article, a series of plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves, are described and compared to conventional binary vectors for stable transformation such as transformation selection genes.
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NAC transcription factors: structurally distinct, functionally diverse
TL;DR: The biological and molecular functions of the NAC family are summarized, paying particular attention to the intricate regulation of NAC protein level and localization, and to the first indications of Nac participation in transcription factor networks.
Journal ArticleDOI
Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression.
Hiroshi Abe,Kazuko Yamaguchi-Shinozaki,Takeshi Urao,Toshisuke Iwasaki,Daijiro Hosokawa,Kazuo Shinozaki +5 more
TL;DR: Results indicate that both the rd22BP1 (MYC) and ATMYB2 (MYB) proteins function as transcriptional activators in the dehydration- and ABA-inducible expression of the r d22 gene.
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