Journal ArticleDOI
Absorption and fluorescence properties of fluorescein
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In this article, the authors characterized the protolytic equilibria of fluorescein and determined the spectroscopic properties of its proclivity to fluorescence, and derived the equilibrium constants relating the chemical activities of the cation, neutral form, anion and dianion.About:
This article is published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.The article was published on 1995-06-01. It has received 951 citations till now. The article focuses on the topics: Quantum yield.read more
Citations
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Characterization of Fluorescein-Oligonucleotide Conjugates and Measurement of Local
Jan Nygren,Mikael Kubista +1 more
TL;DR: In this article, the properties of fluorescein are substantially altered upon conjugation to nucleic acids, affecting not only the molar absorptivities and fluorescence quantum yields but also the protolytic equilibrium constant, fluorescence lifetimes.
Book ChapterDOI
In Situ Imaging of Zinc with Synthetic Fluorescent Probes
Jiyao Yu,Christoph J. Fahrni +1 more
TL;DR: This chapter offers an overview of the most widely employed fluorogenic and ratiometric probes for the detection of Zn(II) in a biological environment, discusses concepts relevant for the design and application of ZN(II)-responsive probes for two-photon excitation microscopy, and outlines current challenges and limitations of their application in biological systems.
DissertationDOI
Light to Electrons to Bonds: Imaging Water Splitting and Collecting Photoexcited Electrons
TL;DR: In this article, the diffusion length of photoexcited electron transport and collection in semiconductor photoelectrodes was measured using optical microscopy, and the effect of photon polarization on emission yield was experimentally tested using gold nanoantennas buried in silicon.
Journal ArticleDOI
BMeS-p-A succinimidyl ester as a sulfonyl aniline dye labeling reagent
TL;DR: In this paper , the authors describe the synthesis and properties of BMeS-p-A-NHS as a sulfonylaniline-based fluorescent labeling reagent, which is suitable for excitation with a versatile 405nm laser diode, emitting green fluorescence with a large Stokes shift at 527 nm.
Access to intrinsically glucoside-based microspheres with boron affinity
Andrew P. Vogt,Thomas Tischer,Udo Geckle,Alexandra M. Greiner,Vanessa Trouillet,Michael Kaupp,Leonie Barner,Thorsten Hofe,Christopher Barner-Kowollik +8 more
TL;DR: A novel boronic acid fluorescent molecule was subsequently conjugated to the microspheres in an aqueous environment, exhibiting the spatial and uniform distribution of glucoside as well as the affinity of themicrospheres to bind with boron, evidenced via fluorescence spectroscopy measurements.
References
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Journal ArticleDOI
Fluorescence detection in automated DNA sequence analysis
Lloyd M. Smith,Jane Z. Sanders,Robert J. Kaiser,Peter Hughes,Chris Dodd,Charles R. Connell,Cheryl Heiner,Stephen B. H. Kent,Leroy Hood +8 more
TL;DR: A method for the partial automation of DNA sequence analysis by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymaticDNA sequence analysis.
Journal ArticleDOI
Determination of the absolute quantum yield of fluorescent solutions
Gregorio Weber,F. W. J. Teale +1 more
Journal ArticleDOI
Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence
TL;DR: The alanine detection limit corresponds to fewer than 6000 molecules injected onto the column and represents an improvement of four orders of magnitude in the state of the art for fluorescent detection of amino acids and an improvement for the detection limit for isothiocyanate derivatives of amino amino acids.