Journal ArticleDOI
Absorption and fluorescence properties of fluorescein
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TLDR
In this article, the authors characterized the protolytic equilibria of fluorescein and determined the spectroscopic properties of its proclivity to fluorescence, and derived the equilibrium constants relating the chemical activities of the cation, neutral form, anion and dianion.About:
This article is published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.The article was published on 1995-06-01. It has received 951 citations till now. The article focuses on the topics: Quantum yield.read more
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The real-time polymerase chain reaction
Mikael Kubista,José Manuel Andrade,Martin Bengtsson,Amin Forootan,Jiri Jonák,Kristina Lind,Radek Sindelka,Robert Sjöback,Björn Sjögreen,Linda Strömbom,Anders Ståhlberg,Neven Zoric +11 more
TL;DR: The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR, a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the mid 80:ies.
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Fluorescent detection of zinc in biological systems: recent development on the design of chemosensors and biosensors
Pengju Jiang,Zijian Guo +1 more
TL;DR: In this article, the progress made in the past decade or so, focusing on sensor design strategy based on molecular structure and fluorescent mechanism is reviewed, and the results show that fluorescent imaging has proven to be the most suitable technique for its in vivo monitoring.
Journal ArticleDOI
Thioflavin T as an Efficient Inducer and Selective Fluorescent Sensor for the Human Telomeric G-Quadruplex DNA
Jyotirmayee Mohanty,Nilotpal Barooah,V. Dhamodharan,S. Harikrishna,Pushpangadan Indira Pradeepkumar,Achikanath C. Bhasikuttan +5 more
TL;DR: The application of a water-soluble fluorogenic dye, Thioflavin T (ThT), in a dual role of exclusively inducing quadruplex folding in the 22AG human telomeric DNA, both in the presence and absence of Tris buffer/salt, is demonstrated.
Journal ArticleDOI
Laser-induced release of encapsulated materials inside living cells
Andre G. Skirtach,Almudena Muñoz Javier,Oliver Kreft,Karen Köhler,Alicia Piera Alberola,Helmuth Möhwald,Wolfgang J. Parak,Gleb B. Sukhorukov +7 more
TL;DR: It is shown that polyelectrolyte-multilayer capsules containing metallic nanoparticles in their walls can be remotely activated to release encapsulated materials inside living cells and this approach is ideally suited to applications where precise control is necessary.
Journal ArticleDOI
Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes
TL;DR: Fluorescence lifetimes of five representative xanthene dye species were measured in HzO, D20 and in a series of alcohol solvents ranging from methanol to octanol as discussed by the authors.
References
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Quantitative spectral analysis of multicomponent equilibria
TL;DR: In this paper, a generalization of a method for the determination of equilibrium constants for binary mixtures is presented, which is applicable to all spectroscopic techniques that provide a linear response, opening up new possibilities to analyze complex chemical systems.
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A new method for the analysis of correlated data using procrustes rotation which is suitable for spectral analysis
TL;DR: Kubista et al. as mentioned in this paper proposed a singular value decomposition of the data and the relation of the sets through Procrustes rotation, which is suitable for spectral analysis.
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Irreversible Photobleaching of the Solutions of Fluorescent Dyes. VI. Photobleaching of Uranin in Alkaline Solutions
TL;DR: In this article, the same conclusions as for eosin and erythrosin, concerning the primary processes both in aqueous and in alcoholic media, were made concerning uranin photobleaching.
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Colloidal Silica as a Standard for Measuring Absolute Fluorescence Yield.
David M. Hercules,Henry Frankel +1 more
TL;DR: The use of colloidal silica as a standard for fluorescence efficiency measurements is proposed because of its advantages over glycogen as aStandard, availability, purity, and stability.