Journal ArticleDOI
Absorption and fluorescence properties of fluorescein
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In this article, the authors characterized the protolytic equilibria of fluorescein and determined the spectroscopic properties of its proclivity to fluorescence, and derived the equilibrium constants relating the chemical activities of the cation, neutral form, anion and dianion.About:
This article is published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.The article was published on 1995-06-01. It has received 951 citations till now. The article focuses on the topics: Quantum yield.read more
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A compact, low-cost and high sensitive LIF based on pinhole metal-capillary and direct laser-diode excitation
Weicheng Cai,Hui Huang,Zhibo Yang,Ruichen Shang,Li Xuejing,Chi Ma,Jian Zhao,Liu Pengbo,Jian Wang,Wang Wei +9 more
TL;DR: In this article, an axial-excitation laser-induced fluorescence (LIF) based on pinhole metal-capillary (PMC) and direct laser-diode excitation was proposed.
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Improved Characterization of the Solution Kinetics and Thermodynamics of Biotin, Biocytin and HABA Binding to Avidin and Streptavidin
Roberto F. Delgadillo,Roberto F. Delgadillo,Timothy C. Mueser,Kathia Zaleta-Rivera,Katie A. Carnes,José González-Valdez,Lawrence J. Parkhurst +6 more
TL;DR: A simple reaction model with a single transition state for non-immobilized reactants whose forward thermodynamic parameters complete the thermodynamic cycle in agreement with previously reported studies is proposed.
Journal ArticleDOI
Silica particles with fluorescein-labelled cores for evaluating accessibility through fluorescence quenching by copper
TL;DR: It was observed that the introduction of well-defined mesopores by pseudomorphic transformation in the presence of a structure-directing agent reduces the core accessibility, and the analysis by conventional gas sorption methods paints an incomplete picture of the mesoporous structure.
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Membrane permeability differentiation at the lipid divide
U. Lapinska,Zehra Kahveci,N. Irwin,David S. Milner,Alyson E. Santoro,Thomas A. Richards,Stefano Pagliara +6 more
TL;DR: In this paper, the authors developed a new approach for assessing the membrane permeability of cell-sized unilamellar vesicles, consisting of an aqueous medium enclosed by a single lipid bilayer.
References
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Fluorescence detection in automated DNA sequence analysis
Lloyd M. Smith,Jane Z. Sanders,Robert J. Kaiser,Peter Hughes,Chris Dodd,Charles R. Connell,Cheryl Heiner,Stephen B. H. Kent,Leroy Hood +8 more
TL;DR: A method for the partial automation of DNA sequence analysis by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymaticDNA sequence analysis.
Journal ArticleDOI
Determination of the absolute quantum yield of fluorescent solutions
Gregorio Weber,F. W. J. Teale +1 more
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Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence
TL;DR: The alanine detection limit corresponds to fewer than 6000 molecules injected onto the column and represents an improvement of four orders of magnitude in the state of the art for fluorescent detection of amino acids and an improvement for the detection limit for isothiocyanate derivatives of amino amino acids.