Journal ArticleDOI
Absorption and fluorescence properties of fluorescein
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In this article, the authors characterized the protolytic equilibria of fluorescein and determined the spectroscopic properties of its proclivity to fluorescence, and derived the equilibrium constants relating the chemical activities of the cation, neutral form, anion and dianion.About:
This article is published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.The article was published on 1995-06-01. It has received 951 citations till now. The article focuses on the topics: Quantum yield.read more
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Dynamic Measurement of Nanoflows: Realization of an Optofluidic Flow Meter to the Nanoliter-per-Minute Scale.
TL;DR: An optofluidic measurement system that can deliver and record light in a precise interrogation region of a microfluidic channel and provides an independent method for determining nonzero flow rates based on a robust scaling relationship between the fluorescence emission and flow.
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A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules.
TL;DR: A novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell is synthesized and displayed intracellular specific fluorescence imaging with low background signals.
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Fluorescence-Based Observation of Transient Electrochemical and Electrokinetic Effects at Nanoconfined Bipolar Electrodes
Karen Scida,Alexander Eden,Netzahualcóyotl Arroyo-Currás,Sean Mackenzie,yesil satik,Carl D. Meinhart,Jan C.T. Eijkel,Sumita Pennathur +7 more
TL;DR: This work demonstrates that the removal of an electric field produces solution-phase charge imbalances in the vicinity of the BPE poles, and dissects the ion transport events that occur in the aftermath of field-induced polarization.
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Bias in the absorption coefficient determination of a fluorescent dye, standard reference material 1932 fluorescein solution
Paul C. DeRose,Gary W. Kramer +1 more
TL;DR: The absorption coefficient of standard reference material ® (SRM ® ) 1932, fluorescein in a borate buffer solution (pH=9.5) has been determined at λ =488.0, 490.5 and 491.0 µm using the US national reference UV/visible spectrophotometer as mentioned in this paper.
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Noninvasive Detection of Mitochondrial Dysfunction in Ocular Hypertension and Primary Open-angle Glaucoma.
Lawrence S. Geyman,Yanin Suwan,Reena Garg,Matthew G. Field,Brian D Krawitz,Brian D Krawitz,Shelley Mo,Shelley Mo,Alexander Pinhas,Alexander Pinhas,Robert Ritch,Richard B Rosen,Richard B Rosen +12 more
TL;DR: Despite lacking clinical evidence of glaucomatous deterioration, OHT eyes displayed significantly elevated macular FPF, suggesting that mitochondrial dysfunction may be detected before structural changes visible on current clinical imaging.
References
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Fluorescence detection in automated DNA sequence analysis
Lloyd M. Smith,Jane Z. Sanders,Robert J. Kaiser,Peter Hughes,Chris Dodd,Charles R. Connell,Cheryl Heiner,Stephen B. H. Kent,Leroy Hood +8 more
TL;DR: A method for the partial automation of DNA sequence analysis by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymaticDNA sequence analysis.
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Determination of the absolute quantum yield of fluorescent solutions
Gregorio Weber,F. W. J. Teale +1 more
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Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence
TL;DR: The alanine detection limit corresponds to fewer than 6000 molecules injected onto the column and represents an improvement of four orders of magnitude in the state of the art for fluorescent detection of amino acids and an improvement for the detection limit for isothiocyanate derivatives of amino amino acids.